Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fukai, S.; Nureki, O.; Sekine, S.; Shimada, A.; Tao, J.; Vassylyev, D.G.; Yokoyama, S.
    Structural basis for double-sieve discrimination of L-valine from L-isoleucine and L-threonine by the complex of tRNA(Val) and valyl-tRNA synthetase (2000), Cell, 103, 793-803.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.9 enzyme in complex with tRNAValCAC isoacceptor and an analogue of the Val-adenylate intermediate, hanging-drop vapour diffusion method, 2 months, 4°C, 7-10 mg/ml protein/tRNA/Val-AMS solution in a molar ration of 1:1.1:2, plus equal volume of crystallization solution containing 50 mM sodium cacodylate, pH 6.5, 1.0 ammonium sulfate, 10 mM MgSO4, 6% 1,8-diaminooctane, equilibration against a reservoir solution of 50 mM sodium cacodylate, pH 6.5, 2.8 M ammonium sulfate, 10 mM MgSO4, X-ray structure determination at 2.9 A resolution and analysis Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal Thermus thermophilus
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Thermus thermophilus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.9 ATP + L-valine + tRNAVal = AMP + diphosphate + L-valyl-tRNAVal pre- and post-transfer editing mechanism, mechanism, active site/editing site Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal
-
 Thermus thermophilus AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal the enzyme is strictly specific for the cognate L-valine and discriminates from the larger L-isoleucine and L-threonine by the tRNA-dependent double sieve mechanism Thermus thermophilus AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.9 G7a
-
 Thermus thermophilus
6.1.1.9 Synthetase, valyl-transfer ribonucleate
-
 Thermus thermophilus
6.1.1.9 Valine transfer ribonucleate ligase
-
 Thermus thermophilus
6.1.1.9 Valine translase
-
 Thermus thermophilus
6.1.1.9 Valine--tRNA ligase
-
 Thermus thermophilus
6.1.1.9 ValRS
-
 Thermus thermophilus
6.1.1.9 Valyl transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.9 Valyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.9 Valyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.9 Valyl-tRNA ligase
-
 Thermus thermophilus
6.1.1.9 Valyl-tRNA synthetase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.9 ATP
-
 Thermus thermophilus