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Literature summary extracted from
- Purification of human von Willebrand factor-cleaving protease and its identification as a new member of the metalloproteinase family (2001), Blood, 98, 1662-1666.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.87 | Urea | activation, substrate is degraded at 1 M | Homo sapiens |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.87 | extracellular | plasma | Homo sapiens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.87 | Ca2+ | dependent on | Homo sapiens | |
| 3.4.24.87 | additional information | enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.87 | 150000 | - |
1 * 150000, SDS-PAGE | Homo sapiens |
| 3.4.24.87 | 150000 | 190000 | gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.87 | proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
| 3.4.24.87 | von Willebrand factor + H2O | Homo sapiens | cleavage of peptide bond Tyr842-Met843 | 2 peptides of 140 kD and 65 kD | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.87 | Homo sapiens | - |
enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.87 | from commercial factor VIII/vWF concentrate | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.24.87 | The enzyme cleaves the von Willebrand factor at bond Tyr842-/-Met843 within the A2 domain | active site sequence HEIGHSFGLEHE 150 amino acid residues from the N-terminus of the protein | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.87 | blood | - |
Homo sapiens | - |
| 3.4.24.87 | plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.87 | proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
| 3.4.24.87 | von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843 | Homo sapiens | 2 peptides of 140 kD and 65 kD | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.87 | monomer | 1 * 150000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.87 | M12.241 | Merops-ID | Homo sapiens |
| 3.4.24.87 | vWF-cleaving protease | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.87 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.87 | 8 | - |
assay at | Homo sapiens |
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