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Literature summary extracted from

  • Zhang, J.; Liu, J.; Zhou, J.; Ren, Y.; Dai, X.; Xiang, H.
    Thermostable esterase from Thermoanaerobacter tengcongensis: high-level expression, purification and characterization (2003), Biotechnol. Lett., 25, 1463-1467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 overexpression in Escherichia coli BL21(DE3) Caldanaerobacter subterraneus subsp. tengcongensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Cu2+ 45% reduced activity Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 deoxycholate 50% inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 additional information no inhibition by 1% Triton X-100 and !% CHAPS, not affected by Ca2+, Mg2+, Mn2+, Li+, Na+, K+, EDTA, and PMSF Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 SDS almost complete inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 Tween 20 almost complete inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 Zn2+ 59% reduced activity Caldanaerobacter subterraneus subsp. tengcongensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 additional information not affected by Ca2+, Mg2+, Mn2+, Li+, Na+, K+, and EDTA, enzyme is no metalloenzyme Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 43000
-
 recombinant enzyme, gel filtration Caldanaerobacter subterraneus subsp. tengcongensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Caldanaerobacter subterraneus subsp. tengcongensis
-
 gene lipA encodes a thermostable esterase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant from Escherichia coli, to homogeneity Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O 4-nitrophenyl propionate and 4-nitrophenyl butyrate are the best substrates Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + laurate
-
 ?
3.1.1.1 4-nitrophenyl palmitate + H2O low activity Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + palmitate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O 4-nitrophenyl propionate and 4-nitrophenyl butyrate are the best substrates Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 43000, recombinant enzyme, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 70
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 75
-
 optimal growth temperature in vivo Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50
-
 10 h, remaining activity more than 80% Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.1 70
-
 1.5 h, 50% remaining activity Caldanaerobacter subterraneus subsp. tengcongensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 8 10
-
 Caldanaerobacter subterraneus subsp. tengcongensis