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Literature summary extracted from

  • Sehgal, A.C.; Tompson, R.; Cavanagh, J.; Kelly, R.M.
    Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1 (2002), Biotechnol. Bioeng., 80, 784-793.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 DMSO optimal activation at 3.5% v/v with an increase in kcat of 20-30%, no dramatic changes in conformation of the enzyme at 3.5% v/v DMSO and 50-80°C, but significant changes at small DMSO concentrations Saccharolobus solfataricus

Application

EC Number Application Comment Organism
3.1.1.1 biotechnology the enzyme can be used as a biocatalyst at suboptimal temperatures through addition of DMSO as an activator Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 acetonitrile inactivation Saccharolobus solfataricus
3.1.1.1 Dimethyl formamide inactivation Saccharolobus solfataricus
3.1.1.1 dioxane inactivation Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 additional information
-
 additional information kinetics Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 acetonitrile inactivation Saccharolobus solfataricus
3.1.1.1 dimethyl formamide inactivation Saccharolobus solfataricus
3.1.1.1 dioxane inactivation Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus
-
 enzyme Est1
-
3.1.1.1 Saccharolobus solfataricus P1
-
 enzyme Est1
-

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 Est1
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 50 80
-
 Saccharolobus solfataricus