BRENDA - Enzyme Database

Isolation from Nocardioides sp. strain CT16, purification, and characterization of a deoxycytidine deaminase extremely thermostable in the presence of DL-dithiothreitol

Sakai, T.; Daikai, T.; Monma, H.; Maeda, H.; Biosci. Biotechnol. Biochem. 66, 1646-1651 (2002)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.055
-
dCMP
pH 7.0, 37C
Nocardioides sp.
3.5.4.5
0.13
-
5-methyl-dCMP
pH 7.0, 37C
Nocardioides sp.
3.5.4.5
0.14
-
CMP
pH 7.0, 37C
Nocardioides sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
18000
-
2 * 18000, SDS-PAGE
Nocardioides sp.
3.5.4.5
52000
-
gel filtration
Nocardioides sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Nocardioides sp.
-
-
-
3.5.4.5
Nocardioides sp. CT16
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Nocardioides sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.4.5
225
-
pH 7.0, 37C
Nocardioides sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methyl-dCMP + H2O
-
650635
Nocardioides sp.
5-methyl-dCyd
-
650635
Nocardioides sp.
?
3.5.4.5
5-methyl-dCMP + H2O
-
650635
Nocardioides sp. CT16
5-methyl-dCyd
-
650635
Nocardioides sp. CT16
?
3.5.4.5
CTP + H2O
-
650635
Nocardioides sp.
UTP + NH3
-
650635
Nocardioides sp.
?
3.5.4.5
CTP + H2O
-
650635
Nocardioides sp. CT16
UTP + NH3
-
650635
Nocardioides sp. CT16
?
3.5.4.5
dCTP + H2O
-
650635
Nocardioides sp.
dUTP + NH3
-
650635
Nocardioides sp.
?
3.5.4.5
dCTP + H2O
-
650635
Nocardioides sp. CT16
dUTP + NH3
-
650635
Nocardioides sp. CT16
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 18000, SDS-PAGE
Nocardioides sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.5
99
-
maximum of activity
Nocardioides sp.
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.4.5
50
-
15 min, absence of dithiothreitol, 44% residual activity
Nocardioides sp.
3.5.4.5
100
-
15 min, 1 mM dithiothreitol, 80% residual activity; 1 h, 1 mM dithiothreitol, 48% residual activity; 1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity; 2 h, 50 mM dithiothreitol, 56% residual activity
Nocardioides sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.055
-
dCMP
pH 7.0, 37C
Nocardioides sp.
3.5.4.5
0.13
-
5-methyl-dCMP
pH 7.0, 37C
Nocardioides sp.
3.5.4.5
0.14
-
CMP
pH 7.0, 37C
Nocardioides sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
18000
-
2 * 18000, SDS-PAGE
Nocardioides sp.
3.5.4.5
52000
-
gel filtration
Nocardioides sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Nocardioides sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.4.5
225
-
pH 7.0, 37C
Nocardioides sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methyl-dCMP + H2O
-
650635
Nocardioides sp.
5-methyl-dCyd
-
650635
Nocardioides sp.
?
3.5.4.5
5-methyl-dCMP + H2O
-
650635
Nocardioides sp. CT16
5-methyl-dCyd
-
650635
Nocardioides sp. CT16
?
3.5.4.5
CTP + H2O
-
650635
Nocardioides sp.
UTP + NH3
-
650635
Nocardioides sp.
?
3.5.4.5
CTP + H2O
-
650635
Nocardioides sp. CT16
UTP + NH3
-
650635
Nocardioides sp. CT16
?
3.5.4.5
dCTP + H2O
-
650635
Nocardioides sp.
dUTP + NH3
-
650635
Nocardioides sp.
?
3.5.4.5
dCTP + H2O
-
650635
Nocardioides sp. CT16
dUTP + NH3
-
650635
Nocardioides sp. CT16
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 18000, SDS-PAGE
Nocardioides sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.5
99
-
maximum of activity
Nocardioides sp.
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.4.5
50
-
15 min, absence of dithiothreitol, 44% residual activity
Nocardioides sp.
3.5.4.5
100
-
15 min, 1 mM dithiothreitol, 80% residual activity; 1 h, 1 mM dithiothreitol, 48% residual activity; 1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity; 2 h, 50 mM dithiothreitol, 56% residual activity
Nocardioides sp.