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Literature summary extracted from

  • Casey, A.; Walsh, G.
    Purification and characterization of extracellular phytase from Aspergillus niger ATCC 9142 (2003), Bioresour. Technol., 86, 183-188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 F- 200 mM, activation to 160.7% of control Aspergillus niger
3.1.3.26 F- 200 mM, activation to 160.7% of control Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ca2+ 200 mM, 32% inhibition Aspergillus niger
3.1.3.26 Ca2+ 200 mM, 32% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.1
-
myo-inositol hexakisphosphate
-
Aspergillus niger
3.1.3.26 0.1
-
myo-inositol hexakisphosphate
-
Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
Aspergillus niger
-
-
3.1.3.26 extracellular
-
Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Cd2+ 200 mM, activation to 156% of control Aspergillus niger
3.1.3.8 Cu2+ 200 mM, activation to 160% of control Aspergillus niger
3.1.3.8 Hg2+ 200 mM, activation to 158% of control Aspergillus niger
3.1.3.8 Mg2+ 200 mM, activation to 154% of control Aspergillus niger
3.1.3.8 Mn2+ 200 mM, activation to 147.8% of control Aspergillus niger
3.1.3.8 additional information no effect is observed after addition of 200 mM Fe2+ or Fe3+ Aspergillus niger
3.1.3.8 Zn2+ 200 mM, activation to 142.7% of control Aspergillus niger
3.1.3.26 Cd2+ 200 mM, activation to 156% of control Aspergillus niger
3.1.3.26 Cu2+ 200 mM, activation to 160% of control Aspergillus niger
3.1.3.26 Hg2+ 200 mM, activation to 158% of control Aspergillus niger
3.1.3.26 Mg2+ 200 mM, activation to 154% of control Aspergillus niger
3.1.3.26 Mn2+ 200 mM, activation to 147.8% of control Aspergillus niger
3.1.3.26 additional information no effect is observed after addition of 200 mM Fe2+ or Fe3+ Aspergillus niger
3.1.3.26 Zn2+ 200 mM, activation to 142.7% of control Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 84000
-
1 * 84000, SDS-PAGE Aspergillus niger
3.1.3.8 87000
-
gel filtration Aspergillus niger
3.1.3.26 84000
-
1 * 84000, SDS-PAGE Aspergillus niger
3.1.3.26 87000
-
gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus niger
-
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein 31% carbohydrate Aspergillus niger
3.1.3.26 glycoprotein 31% carbohydrate Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
Aspergillus niger
3.1.3.26
-
Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 5.276
-
-
Aspergillus niger
3.1.3.26 5.276
-
-
Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O 74% of the activity with myo-inositol hexakisphosphate Aspergillus niger 1-naphthol + phosphate
-
?
3.1.3.8 2-naphthyl phosphate + H2O 63.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger 2-naphthol + phosphate
-
?
3.1.3.8 4-nitrophenyl phosphate + H2O 39.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger 4-nitrophenol + phosphate
-
?
3.1.3.8 ADP + H2O 25.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger ?
-
?
3.1.3.8 AMP + phosphate 43.9% of the activity with myo-inositol hexakisphosphate Aspergillus niger adenosine + phosphate
-
?
3.1.3.8 ATP + H2O 38.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger ?
-
?
3.1.3.8 D-glucose 1-phosphate + H2O 28.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger D-glucose + phosphate
-
?
3.1.3.8 glycerol 2-phosphate + H2O 50.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger glycerol + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger ? + phosphate
-
?
3.1.3.8 phenyl phosphate + H2O 84% of the activity with myo-inositol hexakisphosphate Aspergillus niger phenol + phosphate
-
?
3.1.3.26 1-naphthyl phosphate + H2O 74% of the activity with myo-inositol hexakisphosphate Aspergillus niger 1-naphthol + phosphate
-
?
3.1.3.26 2-naphthyl phosphate + H2O 63.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger 2-naphthol + phosphate
-
?
3.1.3.26 4-nitrophenyl phosphate + H2O 39.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger 4-nitrophenol + phosphate
-
?
3.1.3.26 ADP + H2O 25.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger ?
-
?
3.1.3.26 AMP + phosphate 43.9% of the activity with myo-inositol hexakisphosphate Aspergillus niger adenosine + phosphate
-
?
3.1.3.26 ATP + H2O 38.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger ?
-
?
3.1.3.26 D-glucose 1-phosphate + H2O 28.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger D-glucose + phosphate
-
?
3.1.3.26 glycerol 2-phosphate + H2O 50.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger glycerol + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger ? + phosphate
-
?
3.1.3.26 phenyl phosphate + H2O 84% of the activity with myo-inositol hexakisphosphate Aspergillus niger phenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 84000, SDS-PAGE Aspergillus niger
3.1.3.26 monomer 1 * 84000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 65
-
-
Aspergillus niger
3.1.3.26 65
-
-
Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 45 75 45°C: about 45% of maximal activity, 75°C: about 50% of maximal activity Aspergillus niger
3.1.3.26 45 75 45°C: about 45% of maximal activity, 75°C: about 50% of maximal activity Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 80
-
3 min, 78% loss of activity Aspergillus niger
3.1.3.26 80
-
3 min, 78% loss of activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
and a second lower pH-optimum at pH 11.5 Aspergillus niger
3.1.3.8 11.5
-
and a second higher pH-optimum at pH 11.5 Aspergillus niger
3.1.3.26 5
-
and a second lower pH-optimum at pH 11.5 Aspergillus niger
3.1.3.26 11.5
-
and a second higher pH-optimum at pH 5.0 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2 12 pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity Aspergillus niger
3.1.3.26 2 12 pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity Aspergillus niger