BRENDA - Enzyme Database

Mechanism of 3-methylaspartase probed using deuterium and solvent isotope effects and active-site directed reagents: identification of an essential cysteine residue

Pollard, J.R.; Richardson, S.; Akhtar, M.; Lasry, P.; Neal, T.; Botting, N.P.; Gani, D.; Bioorg. Med. Chem. 7, 949-975 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
4-nitrophenylhydrazine
90% inhibition after 200 min
Clostridium tetanomorphum
4.3.1.2
methylhydrazine
-
Clostridium tetanomorphum
4.3.1.2
N-ethylmaleimide
1 mM, complete inactivation after approx. 12 min
Clostridium tetanomorphum
4.3.1.2
phenylhydrazine
20 mM, complete inhibition after 20 min
Clostridium tetanomorphum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
5.2
-
(2S,3R)-3-methylaspartate
30C, pH 9.0, at 50 mM K+
Clostridium tetanomorphum
4.3.1.2
40
-
(2S,3R)-3-methylaspartate
30C, pH 9.0, at 1 mM K+
Clostridium tetanomorphum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
required for activity
Clostridium tetanomorphum
4.3.1.2
Mg2+
required for activity
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
mesaconate + NH3
-
Clostridium tetanomorphum H1 / ATCC 15920
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Clostridium tetanomorphum
-
-
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
native and recombinant 3-methylaspartase
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
erythro-3-methylaspartate
-
650542
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
650542
Clostridium tetanomorphum
mesaconate + NH3
-
650542
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
4-nitrophenylhydrazine
90% inhibition after 200 min
Clostridium tetanomorphum
4.3.1.2
methylhydrazine
-
Clostridium tetanomorphum
4.3.1.2
N-ethylmaleimide
1 mM, complete inactivation after approx. 12 min
Clostridium tetanomorphum
4.3.1.2
phenylhydrazine
20 mM, complete inhibition after 20 min
Clostridium tetanomorphum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
5.2
-
(2S,3R)-3-methylaspartate
30C, pH 9.0, at 50 mM K+
Clostridium tetanomorphum
4.3.1.2
40
-
(2S,3R)-3-methylaspartate
30C, pH 9.0, at 1 mM K+
Clostridium tetanomorphum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
required for activity
Clostridium tetanomorphum
4.3.1.2
Mg2+
required for activity
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
mesaconate + NH3
-
Clostridium tetanomorphum H1 / ATCC 15920
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
native and recombinant 3-methylaspartase
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
erythro-3-methylaspartate
-
650542
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
650542
Clostridium tetanomorphum
mesaconate + NH3
-
650542
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
650542
Clostridium tetanomorphum H1 / ATCC 15920
r