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Literature summary extracted from

  • Rotari, V.I.; Dando, P.M.; Barrett, A.J.
    Legumain forms from plants and animals differ in their specificity (2001), Biol. Chem., 382, 953-959.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 ovocystatin weak inhibition, from egg-white Phaseolus vulgaris
3.4.22.34 ovocystatin from egg-white Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 protein + H2O Phaseolus vulgaris
-
 peptides
-
 ?
3.4.22.34 protein + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Phaseolus vulgaris
-
 purfied enzyme
-
3.4.22.34 Sus scrofa
-
 purified enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 4.5
-
 purified enzyme Phaseolus vulgaris
3.4.22.34 7
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide + H2O
-
 Phaseolus vulgaris acetyl-Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa acetyl-Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O best synthetic substrate Phaseolus vulgaris benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Phaseolus vulgaris benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O best synthetic substrate Sus scrofa benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Phaseolus vulgaris benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Sus scrofa benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Phaseolus vulgaris benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Sus scrofa benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Phaseolus vulgaris benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Sus scrofa benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Phaseolus vulgaris benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Phaseolus vulgaris benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O low activity Sus scrofa benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Phaseolus vulgaris benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 additional information substrate specificity for P3 position residues Phaseolus vulgaris ?
-
 ?
3.4.22.34 additional information substrate specificity for P3 position residues Sus scrofa ?
-
 ?
3.4.22.34 neurotensin + H2O
-
 Phaseolus vulgaris ?
-
 ?
3.4.22.34 neurotensin + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 protein + H2O
-
 Phaseolus vulgaris peptides
-
 ?
3.4.22.34 protein + H2O
-
 Sus scrofa peptides
-
 ?
3.4.22.34 tetanus toxoid C fragment + H2O cleavage sites are at Asn26, Asn97, and Asn372, but not Asn337 Phaseolus vulgaris 3 fragments
-
 ?
3.4.22.34 tetanus toxoid C fragment + H2O cleavage sites are at Asn26, Asn337, and Asn372 Sus scrofa tetanus toxoid fragments
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Asparaginyl endopeptidase
-
 Phaseolus vulgaris
3.4.22.34 Asparaginyl endopeptidase
-
 Sus scrofa
3.4.22.34 Proteinase B
-
 Phaseolus vulgaris
3.4.22.34 Proteinase B
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 30
-
 assay at Phaseolus vulgaris
3.4.22.34 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5
-
 with substrate acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide Phaseolus vulgaris
3.4.22.34 5
-
 with substrate acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide Sus scrofa
3.4.22.34 5.4
-
 with substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide Phaseolus vulgaris
3.4.22.34 5.8
-
 with substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide Sus scrofa