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Literature summary extracted from
- Biochemical and molecular characterization of Staphylococcus simulans lipase (2001), Biochimie, 83, 863-871.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | DNA and amino acid sequence determination, analysis, expression in Escherichia coli DH5alpha | Staphylococcus simulans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | additional information | no inhibition by bile salts or amphiphilic proteins | Staphylococcus simulans |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.3 | extracellular | enzyme is secreted to the culture medium | Staphylococcus simulans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | Ca2+ | activates 1.6fold, not required for expression of the enzyme, optimal at 3 mM | Staphylococcus simulans |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 45000 | - |
4 * 45000, SDS-PAGE | Staphylococcus simulans |
| 3.1.1.3 | 158000 | - |
gel filtration | Staphylococcus simulans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Staphylococcus simulans | Q84EK3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | 397fold, to homogeneity from culture supernatant | Staphylococcus simulans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 1100 | - |
purified enzyme, substrate tributyrin, 3 mM CaCl2 | Staphylococcus simulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | enzyme hydrolyzes triacylglycerols without chain length specificity | Staphylococcus simulans | ? | - |
? | |
| 3.1.1.3 | tributyrin + H2O | - |
Staphylococcus simulans | dibutyrin + butyrate | - |
? | |
| 3.1.1.3 | triolein + H2O | - |
Staphylococcus simulans | diolein + oleate | - |
? | |
| 3.1.1.3 | tripropionin + H2O | - |
Staphylococcus simulans | dipropionin + propionate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | tetramer | 4 * 45000, SDS-PAGE | Staphylococcus simulans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Staphylococcus simulans |
| 3.1.1.3 | SSL | - |
Staphylococcus simulans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 37 | - |
- |
Staphylococcus simulans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 60 | - |
inactivation after a few min | Staphylococcus simulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 8.5 | - |
- |
Staphylococcus simulans |
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