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Literature summary extracted from

  • Wehbi, H.; Feng, J.; Roberts, M.F.
    Water-miscible organic cosolvents enhance phosphatidylinositol-specific phospholipase C phosphotransferase as well as phosphodiesterase activity (2003), Biochim. Biophys. Acta, 1613, 15-27.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.13 dimethylformamide water-miscible, enhances phosphotransferase activity Bacillus thuringiensis
4.6.1.13 Dimethylsulfoxide water-miscible, enhances phosphotransferase activity Bacillus thuringiensis
4.6.1.13 Isopropanol water-miscible, maximum activation at 30%, activates regardless of the type of phosphatidylinositol substrate, enhances phosphotransferase activity Bacillus thuringiensis
4.6.1.13 additional information isopropanol and diheptanoylphosphatidylcholine activate the hydrolytic activity towards 1D-myo-inositol 1,2-cyclic phosphate, PI-PLC exhibits kinetic interfacial activation Bacillus thuringiensis
4.6.1.13 phosphatidylcholine activates Bacillus thuringiensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.13 expression in Escherichia coli MM294 Bacillus thuringiensis

General Stability

EC Number General Stability Organism
4.6.1.13 myo-inositol stabilizes Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.13 myo-inositol weak Bacillus thuringiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.13 additional information Ca2+-independent Bacillus thuringiensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.6.1.13 isopropanol water/isopropanol mixture decreases stability, at 4°C protein secondary structure is stable for at least 1 week in 30% isopropanol, Tm-value for PI-PLC thermal denaturation decreases linearly with added isopropanol, in presence of 30% isopropanol the Tm-value decreases from 53.6 to 30°C, myo-inositol enhances thermostability in isopropanol Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 Bacillus thuringiensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.13 recombinant PI-PLC Bacillus thuringiensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.13 additional information
-
 phosphotransferase activity towards phosphatidylinositol in several aggregation states in the absence and presence of 30% isopropanol Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol aggregated substrate is preferred over monomeric substrate Bacillus thuringiensis 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol a cyclic phosphodiesterase activity of PI-PLC converts 1D-myo-inositol 1,2-cyclic phosphate to inositol 1-phosphate ?
4.6.1.13 additional information not: phosphatidylcholine Bacillus thuringiensis ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.13 25
-
 some thermal unfolding of PI-PLC occurs Bacillus thuringiensis
4.6.1.13 30
-
 Tm-value in presence of 30% isopropanol, myo-inositol enhances thermostability in isopropanol Bacillus thuringiensis
4.6.1.13 53.6
-
 Tm-value in absence of 30% isopropanol Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.13 7.5
-
 assay at Bacillus thuringiensis