EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.3 | additional information | no activation by metal ions | Rhodococcus erythropolis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.13.1.3 | gene dszZ, DNA sequence determination, cooverexpression in Escherichia coli with chaperonin genes groEL and groES at 25°C | Rhodococcus erythropolis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.13.1.3 | C26S | site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant | Rhodococcus erythropolis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.3 | 2,2'-Dihydroxybiphenyl | competitive | Rhodococcus erythropolis | |
3.13.1.3 | 2,3-Dihydroxybiphenyl | competitive | Rhodococcus erythropolis | |
3.13.1.3 | 2-Hydroxybiphenyl | competitive, product inhibition | Rhodococcus erythropolis | |
3.13.1.3 | Ag+ | strong inhibition at 1 mM | Rhodococcus erythropolis | |
3.13.1.3 | Cu2+ | strong inhibition at 1 mM | Rhodococcus erythropolis | |
3.13.1.3 | DTNB | strong inhibition at 1 mM | Rhodococcus erythropolis | |
3.13.1.3 | Hg2+ | strong inhibition at 1 mM | Rhodococcus erythropolis | |
3.13.1.3 | additional information | no inhibition by product sulfite, no inhibition by chelating agents EDTA, 1,10-phenanthroline, and 8-hydroxyquinone | Rhodococcus erythropolis | |
3.13.1.3 | N-ethylmaleimide | strong inhibition at 1 mM | Rhodococcus erythropolis | |
3.13.1.3 | p-chloromercuribenzoate | strong inhibition at 1 mM | Rhodococcus erythropolis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.3 | 0.0082 | - |
2'-hydroxybiphenyl-2-sulfinate | pH 7.0, 28°C | Rhodococcus erythropolis | |
3.13.1.3 | 0.062 | - |
2-phenylbenzenesulfinate | pH 7.0, 28°C | Rhodococcus erythropolis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.3 | additional information | no metal ions required | Rhodococcus erythropolis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.13.1.3 | 40000 | - |
1 * 40000, SDS-PAGE | Rhodococcus erythropolis |
3.13.1.3 | 43000 | - |
gel filtration | Rhodococcus erythropolis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | Rhodococcus erythropolis | - |
2-hydroxybiphenyl + sulfite | - |
? | |
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | Rhodococcus erythropolis KA2-5-1 | - |
2-hydroxybiphenyl + sulfite | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.13.1.3 | Rhodococcus erythropolis | - |
- |
- |
3.13.1.3 | Rhodococcus erythropolis KA2-5-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.13.1.3 | recombinant 7.8fold from Escherichia coli, and native from strain KA2-5-1 160fold, to homogeneity | Rhodococcus erythropolis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O = 2-hydroxybiphenyl + sulfite | Cys26 is essential for activity | Rhodococcus erythropolis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.13.1.3 | 0.18 | - |
purified native enzyme | Rhodococcus erythropolis |
3.13.1.3 | 0.207 | - |
purified recombinant enzyme | Rhodococcus erythropolis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | - |
Rhodococcus erythropolis | 2-hydroxybiphenyl + sulfite | - |
? | |
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | highly specific for | Rhodococcus erythropolis | 2-hydroxybiphenyl + sulfite | - |
? | |
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | - |
Rhodococcus erythropolis KA2-5-1 | 2-hydroxybiphenyl + sulfite | - |
? | |
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate + H2O | highly specific for | Rhodococcus erythropolis KA2-5-1 | 2-hydroxybiphenyl + sulfite | - |
? | |
3.13.1.3 | 2-phenylbenzenesulfinate + H2O | - |
Rhodococcus erythropolis | biphenyl + sulfite | - |
? | |
3.13.1.3 | 2-phenylbenzenesulfinate + H2O | - |
Rhodococcus erythropolis KA2-5-1 | biphenyl + sulfite | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.13.1.3 | monomer | 1 * 40000, SDS-PAGE | Rhodococcus erythropolis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.13.1.3 | 2'-hydroxybiphenyl-2-sulfinate desulfinase | - |
Rhodococcus erythropolis |
3.13.1.3 | DszB | - |
Rhodococcus erythropolis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.1.3 | 35 | - |
recombinant enzyme | Rhodococcus erythropolis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.1.3 | 28 | - |
recombinant enzyme, stable | Rhodococcus erythropolis |
3.13.1.3 | 30 | - |
recombinant enzyme, rapid loss of activity above, inactivation after 4 h | Rhodococcus erythropolis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.13.1.3 | 7.5 | - |
about, recombinant enzyme | Rhodococcus erythropolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.3 | additional information | no cofactor required | Rhodococcus erythropolis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.3 | 0.25 | - |
2-Hydroxybiphenyl | pH 7.0, 28°C, recombinant enzyme | Rhodococcus erythropolis | |
3.13.1.3 | 0.4 | - |
2,3-Dihydroxybiphenyl | pH 7.0, 28°C, recombinant enzyme | Rhodococcus erythropolis | |
3.13.1.3 | 2 | - |
2,2'-Dihydroxybiphenyl | pH 7.0, 28°C, recombinant enzyme | Rhodococcus erythropolis |