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Literature summary extracted from

  • Mori, S.; Yasuda, T.; Takeshita, H.; Nakajima, T.; Nakazato, E.; Mogi, K.; Kaneko, Y.; Kishi, K.
    Molecular, biochemical and immunological analyses of porcine pancreatic DNase I (2001), Biochim. Biophys. Acta, 1547, 275-287.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.1 DNA sequence determination and analysis Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.1 N110S site-directed mutagenesis, exchange mutant has the same sequence as the rat and bovine enzymes and becomes insensitive to low pH like the rat and bovine enzymes Sus scrofa
3.1.21.1 T178S site-directed mutagenesis, similar to the wild-type enzyme in pH-sensitivity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EDTA slight inhibition Sus scrofa
3.1.21.1 EGTA
-
 Sus scrofa
3.1.21.1 additional information no inhibition by G-actin, due to exchange of Y65 to H65 and A114 to S114 compared to the other G-actin-sensitive mammalian enzymes Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Sus scrofa P11936
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 26500fold from pancreas, to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 kidney low activity Sus scrofa
-
3.1.21.1 liver low activity Sus scrofa
-
3.1.21.1 additional information no activity in spleen, heart, lung, stomach, large intestine, cerebrum, cerebellum, submaxillary gland, and thymus Sus scrofa
-
3.1.21.1 pancreas
-
 Sus scrofa
-
3.1.21.1 parotid gland low activity Sus scrofa
-
3.1.21.1 small intestine low activity Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 dsDNA + H2O
-
 Sus scrofa 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 DNase I
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.1 37
-
 assay at Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.1 additional information
-
 sensitive to low pH, Asn110 is responsible Sus scrofa