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Literature summary extracted from
- Cloning, expression and some properties of alpha-carbonic anhydrase from Helicobacter pylori (2001), Biochim. Biophys. Acta, 1544, 55-63.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | alpha-carbonic anhydrase gene cloned and sequenced, truncated gene expressed in Escherichia coli BL21(DE3) | Helicobacter pylori |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.1 | enzyme appears to denature with a midpoint of a structural transition near 2.1 M denaturant guanidine hydrochloride | Helicobacter pylori |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | 2-acetylamido-1,3,4-thiadiazole-5-sulfonamide | acetazolamide | Helicobacter pylori |  |
| 4.2.1.1 | thiocyanate | - |
Helicobacter pylori | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 17 | - |
CO2 | pH 8.9, 25°C | Helicobacter pylori | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | Zn2+ | - |
Helicobacter pylori | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 28280 | - |
amino acid sequence | Helicobacter pylori |
| 4.2.1.1 | 29000 | - |
SDS-PAGE | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | H2CO3 | Helicobacter pylori | - |
CO2 + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Helicobacter pylori | - |
ATCC type strain 628572 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | - |
Helicobacter pylori |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | 2-nitrophenyl acetate + H2O | - |
Helicobacter pylori | 2-nitrophenol + acetate | - |
r | |
| 4.2.1.1 | 4-nitrophenyl acetate + H2O | - |
Helicobacter pylori | 4-nitrophenol + acetate | - |
r | |
| 4.2.1.1 | H2CO3 | - |
Helicobacter pylori | CO2 + H2O | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | alpha-carbonic anhydrase | - |
Helicobacter pylori |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 240000 | - |
CO2 | pH 8.9, 25°C | Helicobacter pylori | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 8.8 | - |
- |
Helicobacter pylori |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 6.2 | 9 | - |
Helicobacter pylori |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 2.8 | - |
thiocyanate | pH 7.5, 25°C | Helicobacter pylori | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.1 | Helicobacter pylori | calculated from amino acid composition | - |
8.8 |
| 4.2.1.1 | Helicobacter pylori | isoelectric focusing | - |
9 |
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