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Literature summary extracted from

  • van Kampen, M.D.; Rosenstein, R.; Gotz, F.; Egmond, M.R.
    Cloning, purification and characterisation of Staphylococcus warneri lipase 2 (2001), Biochim. Biophys. Acta, 1544, 229-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged mature enzyme in Escherichia coli DH5alpha Staphylococcus warneri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 EDTA 3% remaining activity at 5 mM Staphylococcus warneri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Staphylococcus warneri
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ required Staphylococcus warneri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45500
-
 x * 45500, SDS-PAGE Staphylococcus warneri

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Staphylococcus warneri
-
 SWL2, lipase 2
-
3.1.1.3 Staphylococcus warneri 863
-
 SWL2, lipase 2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 additional information enzyme is organized as a prepro-protein Staphylococcus warneri

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant His-tagged enzyme from Escherichia coli Staphylococcus warneri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 14.4
-
 purified recombinant enzyme, substrate 2-hexandecanoylthio-ethane-1-phosphocholine Staphylococcus warneri
3.1.1.3 25
-
 purified recombinant enzyme, substrate 4-nitrophenyl butyrate Staphylococcus warneri
3.1.1.3 115
-
 purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphocholine Staphylococcus warneri
3.1.1.3 123
-
 purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Staphylococcus warneri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Staphylococcus warneri ?
-
 ?
3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Staphylococcus warneri 863 ?
-
 ?
3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
 Staphylococcus warneri ?
-
 ?
3.1.1.3 2-hexandecanoylthio-ethane-1-phosphocholine + H2O
-
 Staphylococcus warneri ?
-
 ?
3.1.1.3 2-hexandecanoylthio-ethane-1-phosphocholine + H2O
-
 Staphylococcus warneri 863 ?
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Staphylococcus warneri 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Staphylococcus warneri 863 4-nitrophenol + butyrate
-
 ?
3.1.1.3 additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri ?
-
 ?
3.1.1.3 additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri 863 ?
-
 ?
3.1.1.3 triolein + H2O low activity Staphylococcus warneri diolein + oleate
-
 ?
3.1.1.3 triolein + H2O low activity Staphylococcus warneri 863 diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 45500, SDS-PAGE Staphylococcus warneri

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase 2
-
 Staphylococcus warneri
3.1.1.3 SWL2
-
 Staphylococcus warneri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
 about, broad optimum Staphylococcus warneri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5.5 9
-
 Staphylococcus warneri