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Literature summary extracted from

  • Masson, P.; Xie, W.; Froment, M.T.; Lockridge, O.
    Effects of mutations of active site residues and amino acids interacting with the W loop on substrate activation of butyrylcholinesterase (2001), Biochim. Biophys. Acta, 1544, 166-176.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 A328F no substrate activation Homo sapiens
3.1.1.8 A328Y no substrate activation Homo sapiens
3.1.1.8 E197D moderately reduced substrate activation Homo sapiens
3.1.1.8 E197G strongly reduced substrate activation Homo sapiens
3.1.1.8 E197Q strongly reduced substrate activation Homo sapiens
3.1.1.8 G328G retained substrate activation Homo sapiens
3.1.1.8 K339M retained substrate activation Homo sapiens
3.1.1.8 N83A loss of substrate activation Homo sapiens
3.1.1.8 N83Q loss of substrate activation Homo sapiens
3.1.1.8 W430A retained substrate activation, strongly reduced affinity for tetramethylammonium, bimolecular rate constant for reaction with diisopropyl fluorophosphate is reduced 10000fold Homo sapiens
3.1.1.8 W82A apparent substrate activation at butytylthiocholine concentrations greater than 2 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 tetraethylammonium
-
 Homo sapiens
3.1.1.8 Tetramethylammonium
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.003
-
 succinylthiocholine wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 0.011
-
 butyrylthiocholine A328Y mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.017
-
 butyrylthiocholine A328F mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.019
-
 succinylthiocholine K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.02
-
 butyrylthiocholine wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 0.028
-
 butyrylthiocholine G328G mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.05
-
 butyrylthiocholine N83A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.06
-
 butyrylthiocholine N83Q and K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.1
-
 butyrylthiocholine E197D and E197Q mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.12
-
 2-Nitrophenyl butyrate wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 0.16
-
 2-Nitrophenyl butyrate K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.2
-
 butyrylthiocholine E197G mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.4
-
 butyrylthiocholine W82A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 1.4
-
 2-Nitrophenyl butyrate W82A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 1.9
-
 2-Nitrophenyl butyrate W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 6
-
 butyrylthiocholine W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 11
-
 succinylthiocholine W430A mutant, pH 7, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 2-nitrophenyl butyrate
-
 Homo sapiens 2-nitrophenol + butyrate
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?
3.1.1.8 succinylthiocholine + H2O
-
 Homo sapiens thiocholine + succinate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 2
-
 butyrylthiocholine W82A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 3
-
 succinylthiocholine K339M and W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 6
-
 succinylthiocholine wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 67
-
 butyrylthiocholine E197D mutant, pH 7, 25°C Homo sapiens
3.1.1.8 83
-
 butyrylthiocholine W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 150
-
 butyrylthiocholine E197Q mutant, pH 7, 25°C Homo sapiens
3.1.1.8 250
-
 butyrylthiocholine K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 250
-
 2-Nitrophenyl butyrate K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 333
-
 butyrylthiocholine G328G mutant, pH 7, 25°C Homo sapiens
3.1.1.8 333
-
 2-Nitrophenyl butyrate W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 416
-
 2-Nitrophenyl butyrate W82A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 450
-
 butyrylthiocholine wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 533
-
 butyrylthiocholine A328F mutant, pH 7, 25°C Homo sapiens
3.1.1.8 600
-
 2-Nitrophenyl butyrate wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 617
-
 butyrylthiocholine N83A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 667
-
 butyrylthiocholine A328Y mutant, pH 7, 25°C Homo sapiens
3.1.1.8 767
-
 butyrylthiocholine N83Q mutant, pH 7, 25°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.8 2
-
 tetraethylammonium wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 4
-
 Tetramethylammonium wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 11
-
 Tetramethylammonium K339M mutant, pH 7, 25°C Homo sapiens
3.1.1.8 70
-
 Tetramethylammonium E197G mutant, pH 7, 25°C Homo sapiens
3.1.1.8 400
-
 tetraethylammonium W82A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 500
-
 Tetramethylammonium W430A mutant, pH 7, 25°C Homo sapiens
3.1.1.8 600
-
 Tetramethylammonium W82A mutant, pH 7, 25°C Homo sapiens