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Literature summary extracted from

  • Kalisz, H.M.; Erck, C.; Plessmann, U.; Wehland, J.
    Incorporation of nitrotyrosine into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase (2000), Biochim. Biophys. Acta, 1481, 131-138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.25 expression in Escherichia coli Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.25 3-nitro-L-tyrosine 50% inhibition of tyrosine incorporation at 50fold higher concentrations of nitrotyrosine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.25 0.0068
-
 L-tyrosine 37°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.25 43000
-
 x * 43000, SDS-PAGE, immunoblot Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-tyrosine Sus scrofa
-
 alpha-tubulin + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.25 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.25 GST-TTL fusion protein, glutathione-sepharose affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
6.3.2.25 -70°C, 25 mM K+-morpholinoethanesulfonic acid, pH 6.8, 150 mM KCl, 12.5 MgCl2, 2.5 mM ATP, 1 mM dithiothreitol, 15% glycerol, at least 4 months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.25 ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine 35fold lower affinity for nitrotyrosine than for L-tyrosine, nitrotyrosine is incoorporated into the C-terminus Sus scrofa nitrotyrosinated alpha-tubulin + ADP + phosphate
-
 ?
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-tyrosine
-
 Sus scrofa alpha-tubulin + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.25 ? x * 43000, SDS-PAGE, immunoblot Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
6.3.2.25 TTL
-
 Sus scrofa
6.3.2.25 tubuline-tyrosine ligase
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.25 0.24
-
 3-nitro-L-tyrosine 37°C Sus scrofa