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Literature summary extracted from

  • Mukhopadhyay, B.; Purwantini, E.
    Pyruvate carboxylase from Mycobacterium smegmatis: stabilization, rapid purification, molecular and biochemical characterization and regulation of the cellular level (2000), Biochim. Biophys. Acta, 1475, 191-206.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetyl-CoA dependent, half maximal activation at 0.014 mM Mycolicibacterium smegmatis
6.4.1.1 butyryl-CoA can replace acetyl-CoA with little efficiency Mycolicibacterium smegmatis
6.4.1.1 propionyl-CoA can replace acetyl-CoA with little efficiency Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.1
-
 Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 ADP
-
 Mycolicibacterium smegmatis
6.4.1.1 ATP
-
 Mycolicibacterium smegmatis
6.4.1.1 Co2+ at excess concentrations Mycolicibacterium smegmatis
6.4.1.1 L-aspartate 47% inhibition at 10 mM Mycolicibacterium smegmatis
6.4.1.1 Mg2+ at excess concentrations Mycolicibacterium smegmatis
6.4.1.1 Mn2+ at excess concentrations Mycolicibacterium smegmatis
6.4.1.1 additional information no inhibitors: glutamate and alpha-ketoglutarate Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.2
-
 ATP 37°C and 45°C, pH 8, Mg2+ concentration higher than ATP concentration Mycolicibacterium smegmatis
6.4.1.1 0.3
-
 pyruvate 37°C, pH 8 Mycolicibacterium smegmatis
6.4.1.1 0.45
-
 pyruvate 45°C, pH 8 Mycolicibacterium smegmatis
6.4.1.1 1.26
-
 ATP 37°C, pH 8, Mg2+ concentration equal to that of ATP Mycolicibacterium smegmatis
6.4.1.1 3
-
 HCO3- 37°C, pH 8 Mycolicibacterium smegmatis
6.4.1.1 4.6
-
 HCO3- 45°C, pH 8 Mycolicibacterium smegmatis
6.4.1.1 9.2
-
 ATP 45°C, pH 8, Mg2+ concentration equal to that of ATP Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 Co2+ can replace Mg2+ with little efficiency Mycolicibacterium smegmatis
6.4.1.1 Mg2+ requirement Mycolicibacterium smegmatis
6.4.1.1 Mn2+ can replace Mg2+ with little efficiency Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 120000
-
 x * 120000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3- Mycolicibacterium smegmatis anaplerotic enzyme ADP + phosphate + oxaloacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 150
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
6.4.1.1 -70°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol Mycolicibacterium smegmatis
6.4.1.1 4°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Mycolicibacterium smegmatis ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- anaplerotic enzyme Mycolicibacterium smegmatis ADP + phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 multimer x * 120000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
6.4.1.1 PC
-
 Mycolicibacterium smegmatis
6.4.1.1 PYC
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin
-
 Mycolicibacterium smegmatis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.4.1.1 2.24
-
 ATP 45°C, pH 8 Mycolicibacterium smegmatis