EC Number | General Stability | Organism |
---|---|---|
4.2.2.13 | 1 mM NaCl or 10 mM calcium acetate stabilize against thermal inactivation at 50°C | Gracilariopsis chorda |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.13 | 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride | - |
Gracilariopsis chorda | |
4.2.2.13 | 2,4,6-trinitrobenzene-sulfonyl acid | - |
Gracilariopsis chorda | |
4.2.2.13 | Cu2+ | 10 mM, complete inactivation | Gracilariopsis chorda | |
4.2.2.13 | Hg2+ | 1 mM, complete inactivation | Gracilariopsis chorda | |
4.2.2.13 | additional information | no effect with 1 mM Mg2+, Zn2+, Fe2+ or Co2+ | Gracilariopsis chorda | |
4.2.2.13 | N-bromosuccinimide | - |
Gracilariopsis chorda |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.13 | 0.03 | - |
amylopectin | pH 5.0, 35°C, barley amylopectin or potato amylopectin | Gracilariopsis chorda | |
4.2.2.13 | 0.03 | - |
glycogen | pH 5.0, 35°C, rabbit liver glycogen | Gracilariopsis chorda | |
4.2.2.13 | 0.1 | - |
maltotetraose | pH 5.0, 35°C | Gracilariopsis chorda | |
4.2.2.13 | 0.1 | - |
maltopentaose | pH 5.0, 35°C | Gracilariopsis chorda | |
4.2.2.13 | 0.1 | - |
maltohexaose | pH 5.0, 35°C | Gracilariopsis chorda | |
4.2.2.13 | 0.1 | - |
maltoheptaose | pH 5.0, 35°C | Gracilariopsis chorda | |
4.2.2.13 | 0.4 | - |
maltotriose | pH 5.0, 35°C | Gracilariopsis chorda | |
4.2.2.13 | 2.3 | - |
maltose | pH 5.0, 35°C | Gracilariopsis chorda |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.13 | Ca2+ | 1.3fold activation | Gracilariopsis chorda |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.13 | 100000 | - |
x * 100000, SDS-PAGE | Gracilariopsis chorda |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.13 | Gracilariopsis chorda | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.13 | - |
Gracilariopsis chorda |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.13 | amylopectin | barley amylopectin and potato amylopectin | Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | Glycogen | rabbit liver glycogen | Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | Maltoheptaose | - |
Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | Maltohexaose | - |
Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | maltopentaose | - |
Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | maltose | - |
Gracilariopsis chorda | D-glucose + 1,5-anhydro-D-fructose | - |
? | |
4.2.2.13 | Maltotetraose | - |
Gracilariopsis chorda | ? | - |
? | |
4.2.2.13 | maltotriose | - |
Gracilariopsis chorda | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.13 | ? | x * 100000, SDS-PAGE | Gracilariopsis chorda |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.13 | GLase | - |
Gracilariopsis chorda |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.13 | 45 | - |
optimal activity without addition of any salt | Gracilariopsis chorda |
4.2.2.13 | 50 | - |
optimal activity in presence of 1 mM calcium chloride | Gracilariopsis chorda |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.13 | 35 | 55 | 35°C: about 50% of maximal activity, 55°C: about 80% of maximal activity, optimal activity in presence of 1 mM calcium chloride | Gracilariopsis chorda |
4.2.2.13 | 35 | 50 | 35°C: about 70% of maximal activity, 50°C: about 70% of maximal activity, activity without addition of any salt | Gracilariopsis chorda |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.13 | 50 | - |
pH 5.0, 20 min, 77% loss of activity without addition of any salt, 49% loss of activity in presence of 1 mM NaCl, 41% loss of activity in presence of 10 mM calcium acetate | Gracilariopsis chorda |