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Literature summary extracted from

  • Stepanov, V.G.; Moor, N.A.; Ankilova, V.N.; Vasil'eva, I.A.; Sukhanova, M.V.; Lavrik, O.I.
    A peculiarity of the reaction of tRNA aminoacylation catalyzed by phenylalanyl-tRNA synthetase from the extreme thermophile Thermus thermophilus (1998), Biochim. Biophys. Acta, 1386, 1-15.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.20 additional information the rate of overcharging of the tRNAPhe is dependenton the buffer system used Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.20 AMP
-
 Thermus thermophilus
6.1.1.20 diphosphate
-
 Thermus thermophilus
6.1.1.20 additional information the rate of overcharging of the tRNAPhe is dependenton the buffer system used Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Thermus thermophilus
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Thermus thermophilus
-
-
-
6.1.1.20 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 0.21
-
 purified enzyme, 37°C Thermus thermophilus
6.1.1.20 0.31
-
 purified recombinant enzyme expressed in Escherichia coli, 37°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + 2 L-phenylalanine + tRNAPhe mechanism, formation of bisphenylalanyl-tRNAPhe with tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human, the second phenylalanyl residue is attached to tRNA approximately 50 times more slowly than the first one, the presence of modified nucleotides is not necessary for tRNAPhe overcharging Thermus thermophilus AMP + diphosphate + bis-L-phenylalanyl-tRNAPhe overcharged product cannot be isolated from living cells ir
6.1.1.20 ATP + 2 L-phenylalanine + tRNAPhe mechanism, formation of bisphenylalanyl-tRNAPhe with tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human, the second phenylalanyl residue is attached to tRNA approximately 50 times more slowly than the first one, the presence of modified nucleotides is not necessary for tRNAPhe overcharging Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + bis-L-phenylalanyl-tRNAPhe overcharged product cannot be isolated from living cells ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 CML33
-
 Thermus thermophilus
6.1.1.20 FRS
-
 Thermus thermophilus
6.1.1.20 HSPC173
-
 Thermus thermophilus
6.1.1.20 L-Phenylalanyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanine translase
-
 Thermus thermophilus
6.1.1.20 Phenylalanine--tRNA ligase
-
 Thermus thermophilus
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-transfer RNA ligase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-tRNA ligase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.20 PheRS
-
 Thermus thermophilus
6.1.1.20 Synthetase, phenylalanyl-transfer ribonucleate
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 40
-
 assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 9
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP
-
 Thermus thermophilus