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Literature summary extracted from
- Guanidine hydrochloride denaturation of glycosylated and deglycosylated stem bromelain (2003), Biochemistry, 68, 1097-1100.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.22.32 | denaturation in guanidine hydrochloride, the deglycosylated enzyme is more sensitive than the glycosylated one, midpoints of transition are at 2.28 M and 2.86 M, respectively | Ananas comosus |
| 3.4.22.32 | the glycosylated enzyme is more stable than the deglycosylated one | Ananas comosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.32 | protein + H2O | Ananas comosus | - |
peptides | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.32 | Ananas comosus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.32 | glycoprotein | N-glycosylated enzyme, glycosylation stabilizes the enzyme | Ananas comosus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.32 | to homogeneity | Ananas comosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.32 | stem | - |
Ananas comosus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.32 | 0.62 | - |
purified enzyme, substrate casein | Ananas comosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.32 | casein + H2O | - |
Ananas comosus | ? | - |
? | |
| 3.4.22.32 | protein + H2O | - |
Ananas comosus | peptides | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.32 | 37 | - |
assay at | Ananas comosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.32 | 7 | - |
assay at | Ananas comosus |
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Release 2023.1 (January 2023)
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