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Literature summary extracted from

  • Goodall, J.J.; Chen, G.J.; Page, M.G.
    Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase (2004), Biochemistry, 43, 4583-4591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 expression in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29 crystal structure at 1.3 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.29 D93N 4% of wild-type kcat Escherichia coli
3.1.1.29 H20N no activity Escherichia coli
3.1.1.29 H20Q no activity Escherichia coli
3.1.1.29 K103Q 54% of wild-type kcat Escherichia coli
3.1.1.29 K103R 68% of wild-type kcat Escherichia coli
3.1.1.29 K103S 28% of wild-type kcat Escherichia coli
3.1.1.29 K105Q 20% of wild-type kcat Escherichia coli
3.1.1.29 K105R 26% of wild-type kcat Escherichia coli
3.1.1.29 K105S 16% of wild-type kcat Escherichia coli
3.1.1.29 K113Q 98% of wild-type kacat Escherichia coli
3.1.1.29 M67E 0.5% of wild-type kcat Escherichia coli
3.1.1.29 N10D 0.05% of wild-type kcat Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 diethyldicarbonate 0.5 mM, 90% inactivation after 10 min, activity can be recovered to 41% of initial activity by treatment wit 200 mM hydroxylamine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 0.0055
-
 Oregon Green-methionine-tRNA 25°C, pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.29 N-substituted aminoacyl-tRNA + H2O Escherichia coli
-
 N-substituted amino acid + tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 recombinant Pth Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 N-substituted aminoacyl-tRNA + H2O
-
 Escherichia coli N-substituted amino acid + tRNA
-
 ?
3.1.1.29 Oregon Green-methionine-tRNA + H2O
-
 Escherichia coli Oregon Green-methionine + tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 PTH
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.29 9.3
-
 Oregon Green-methionine-tRNA 25°C, pH 7.5 Escherichia coli