Literature summary extracted from
Hur, O.; Niks, D.; Casino, P.; Dunn, M.F.
Proton transfers in the beta-reaction catalyzed by tryptophan synthase (2002), Biochemistry, 41, 9991-10001.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
aniline |
nucleophilic analogue used in place of indole leads to formation of intermediate, but no amino acid formation |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
additional information |
- |
additional information |
kinetics of proton release during beta-reaction, thermodynamic cycle of the interconversion of L-serine and alpha-aminoacrylate intermediate structures |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
additional information |
effects of Na+ and Cs+ in the intermediate formation between L-serine and aniline |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.20 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.2.1.20 |
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O |
also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), catalytic mechanism of beta-reaction |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.20 |
1-(indol-3-yl)glycerol 3-phosphate |
alpha-subunit of the bienzyme complex, alpha-reaction |
Salmonella enterica subsp. enterica serovar Typhimurium |
D-glyceraldehyde 3-phosphate + indole |
- |
? |
|
4.2.1.20 |
L-serine + indole |
beta-subunit of the bienzyme complex, beta-reaction, involves multiple covalent transformations facilitated by proton transfer between the cofactor, the reacting substrates, and acid-base catalytic groups of the enzyme |
Salmonella enterica subsp. enterica serovar Typhimurium |
L-tryptophan + H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.1.20 |
tetramer |
alpha2beta2 enzyme complex |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
pyridoxal 5'-phosphate |
forms an alpha-aminoacrylate Schiffbase intermediate with L-serine |
Salmonella enterica subsp. enterica serovar Typhimurium |
|