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Literature summary extracted from

  • Nigou, J.; Dover, L.G.; Besra, G.S.
    Purification and biochemical characterization of Mycobacterium tuberculosis SuhB, an inositol monophosphatase involved in inositol biosynthesis (2002), Biochemistry, 41, 4392-4398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.25 D104N activity almost completely abolished Mycobacterium tuberculosis
3.1.3.25 D107N activity almost completely abolished Mycobacterium tuberculosis
3.1.3.25 D235N activity almost completely abolished Mycobacterium tuberculosis
3.1.3.25 E83D activity almost completely abolished Mycobacterium tuberculosis
3.1.3.25 L81A 10fold more resistant towards Li+ inhibition compared to wild type enzyme Mycobacterium tuberculosis
3.1.3.25 W234L activity almost completely abolished Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Be2+ 50% inhibition at 0.09 mM Mycobacterium tuberculosis
3.1.3.25 Ca2+ 50% inhibition at 0.4 mM Mycobacterium tuberculosis
3.1.3.25 Co2+ 50% inhibition at 0.7 mM Mycobacterium tuberculosis
3.1.3.25 Cr2+ 50% inhibition at 3 mM Mycobacterium tuberculosis
3.1.3.25 Cu2+ 50% inhibition at 1 mM Mycobacterium tuberculosis
3.1.3.25 Li+ 50% inhibition at 0.9 mM Mycobacterium tuberculosis
3.1.3.25 Mn2+ 50% inhibition at 0.8 mM Mycobacterium tuberculosis
3.1.3.25 Ni2+ 50% inhibition at 30 mM Mycobacterium tuberculosis
3.1.3.25 Zn2+ 50% inhibition at 0.005 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.177
-
 myo-inositol 1-phosphate pH 8.5, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Co2+ 30% of the activity with Mg2+ Mycobacterium tuberculosis
3.1.3.25 Fe2+ 30% of the activity with Mg2+ Mycobacterium tuberculosis
3.1.3.25 Mg2+ required, highest activity 6-100 mM Mg2+, inhibitory above 100 mM Mycobacterium tuberculosis
3.1.3.25 Mn2+ 13% of the activity with Mn2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 30000
-
 calculated from amino acid sequence Mycobacterium tuberculosis
3.1.3.25 36000
-
 3 * 36000, SDs-PAGE Mycobacterium tuberculosis
3.1.3.25 93000
-
 native PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol 1-phosphate + H2O Mycobacterium tuberculosis synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 recombinant protein from Escherichia coli using His-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O
-
 Mycobacterium tuberculosis adenosine + phosphate
-
 ?
3.1.3.25 glucitol 6-phosphate + H2O
-
 Mycobacterium tuberculosis glucitol + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Mycobacterium tuberculosis glycerol + phosphate
-
 ?
3.1.3.25 mannitol 1-phosphate + H2O
-
 Mycobacterium tuberculosis mannitol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Mycobacterium tuberculosis myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol Mycobacterium tuberculosis myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 2-phosphate + H2O
-
 Mycobacterium tuberculosis myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 trimer 3 * 36000, SDs-PAGE Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 80
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.25 40 100
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 70
-
 50% of activity lost within 1 min, Mg2+ stabilizes activity for short periods Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.25 3.6
-
 myo-inositol 1-phosphate pH 8.5, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 8.5
-
-
 Mycobacterium tuberculosis