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Literature summary extracted from

  • Bienvenue, D.L.; Gilner, D.; Holz, R.C.
    Hydrolysis of thionopeptides by the aminopeptidase from Aeromonas proteolytica: insight into substrate binding (2002), Biochemistry, 41, 3712-3719.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 1-Phenyl-2-thiourea competitive Vibrio proteolyticus
3.4.11.10 EDTA
-
 Vibrio proteolyticus
3.4.11.10 L-leucine anilide
-
 Vibrio proteolyticus
3.4.11.10 L-leucine anisidide
-
 Vibrio proteolyticus
3.4.11.10 Phenylurea
-
 Vibrio proteolyticus
3.4.11.10 thionoleucine-S-anilide
-
 Vibrio proteolyticus
3.4.11.10 thionoleucine-S-anisidide
-
 Vibrio proteolyticus
3.4.11.10 Urea competitive Vibrio proteolyticus
3.4.11.22 1-Phenyl-2-thiourea competitive Vibrio proteolyticus
3.4.11.22 Urea competitive Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 additional information
-
 additional information kinetics with Zn and/or Cd-containing enzyme, overview Vibrio proteolyticus
3.4.11.10 4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.10 4
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 4.6
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 7
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 7.8
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 8
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.10 10
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 11
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 15
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 32
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 34
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 45
-
 L-leucine anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 50
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 61
-
 L-leucine anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 78
-
 L-leucine anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.22 4
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 4.6
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 7
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 7.8
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 8
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.22 10
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 11
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 15
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 32
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 34
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 45
-
 L-leucin-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 50
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 61
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 78
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Cd2+ the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
3.4.11.10 Cd2+ can substitute for one or both of the Zn2+ ions Vibrio proteolyticus
3.4.11.10 Co2+ Co2+ can substitute for Cd2+ in the bimetallic enzyme Vibrio proteolyticus
3.4.11.10 Co2+ can substitute for one or both of the Zn2+ ions Vibrio proteolyticus
3.4.11.10 additional information bimetallic enzyme, binding conformations, overview Vibrio proteolyticus
3.4.11.10 Zn2+ the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
3.4.11.10 Zn2+ zinc-metallopeptidase, 2 ions per enzyme molecule Vibrio proteolyticus
3.4.11.22 Cd2+ the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
3.4.11.22 Co2+ Co2+ can substitute for Cd2+ in the bimetallic enzyme Vibrio proteolyticus
3.4.11.22 Zn2+ the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Vibrio proteolyticus
-
-
-
3.4.11.22 Vibrio proteolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10
-
 Vibrio proteolyticus
3.4.11.10 native enzyme Vibrio proteolyticus
3.4.11.22
-
 Vibrio proteolyticus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.10 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. substrate binding mechanism and structure Vibrio proteolyticus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Vibrio proteolyticus L-Leu + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Vibrio proteolyticus L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.10 L-leucine 4-nitroanilide + H2O
-
 Vibrio proteolyticus L-leucine + 4-nitroaniline
-
 ?
3.4.11.10 L-leucine anilide + H2O
-
 Vibrio proteolyticus L-leucine + aniline
-
 ?
3.4.11.10 L-leucine-4-anisidide + H2O
-
 Vibrio proteolyticus L-leucine + anisidine
-
 ?
3.4.11.10 thionoleucine-4-anisidide + H2O
-
 Vibrio proteolyticus thionoleucine + anisidine
-
 ?
3.4.11.10 thionoleucine-S-anilide + H2O
-
 Vibrio proteolyticus thionoleucine + aniline
-
 ?
3.4.11.22 L-leucine 4-nitroanilide + H2O
-
 Vibrio proteolyticus L-leucine + 4-nitroaniline
-
 ?
3.4.11.22 L-leucine-4-anisidide + H2O
-
 Vibrio proteolyticus L-leucine + anisidine
-
 ?
3.4.11.22 L-leucine-anilide + H2O
-
 Vibrio proteolyticus L-leucine + aniline
-
 ?
3.4.11.22 thionoleucine-4-anisidide + H2O
-
 Vibrio proteolyticus thionoleucine + anisidine
-
 ?
3.4.11.22 thionoleucine-S-anilide + H2O
-
 Vibrio proteolyticus thionoleucine + aniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 AAP
-
 Vibrio proteolyticus
3.4.11.10 Aeromonas proteolytica aminopeptidase
-
 Vibrio proteolyticus
3.4.11.10 More see also EC 3.4.11.22 Vibrio proteolyticus
3.4.11.22 AAP
-
 Vibrio proteolyticus
3.4.11.22 Aeromonas proteolytica aminopeptidase
-
 Vibrio proteolyticus
3.4.11.22 More see also EC 3.4.11.10 Vibrio proteolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 25
-
 assay at Vibrio proteolyticus
3.4.11.22 25
-
 assay at Vibrio proteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 3.8
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 5.3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 6.3
-
 L-leucine anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 7.5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 7.7
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 7.9
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.10 9.3
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 9.5
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 10.6
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.10 11.1
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 11.5
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 12.2
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 15.1
-
 L-leucine anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.10 16
-
 L-leucine anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.10 16.9
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 23.3
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.10 60
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 3.8
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 5.3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 6.3
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 7.5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 7.7
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 7.9
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.22 9.3
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 9.5
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 10.6
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
3.4.11.22 11.1
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 11.5
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 12.2
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 15.1
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.4.11.22 16
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
3.4.11.22 16.9
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 23.3
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
3.4.11.22 60
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 8
-
 assay at Vibrio proteolyticus
3.4.11.22 8
-
 assay at Vibrio proteolyticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.10 additional information
-
 additional information inhibition kinetics with Zn and/or Cd-containing enzyme, overview Vibrio proteolyticus
3.4.11.10 0.24
-
 1-Phenyl-2-thiourea pH 8.0, 25°C Vibrio proteolyticus
3.4.11.10 4.6
-
 Urea pH 8.0, 25°C Vibrio proteolyticus
3.4.11.22 0.24
-
 1-Phenyl-2-thiourea pH 8.0, 25°C Vibrio proteolyticus
3.4.11.22 4.6
-
 Urea pH 8.0, 25°C Vibrio proteolyticus