Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schmid, M.; Wild, M.R.; Dahinden, P.; Dimroth, P.
    Subunit gamma of the oxaloacetate decarboxylase Na(+) pump: interaction with other subunits/domains of the complex and binding site for the Zn(2+) metal ion (2002), Biochemistry, 41, 1285-1292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.2 expression in Escherichia coli, expression of mutant enzymes and domains of the alpha subunit Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
7.2.4.2 D62A gamma subunit, Zn2+ content decreased to 35% of the wild type enzyme, roughly 30% of activity Klebsiella pneumoniae
7.2.4.2 D63A gamma subunit, almost no effect on activity Klebsiella pneumoniae
7.2.4.2 DELTAH76-P83 gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae
7.2.4.2 DELTAH78-P83 gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae
7.2.4.2 DELTAH82-P83 gamma subunit, Zn2+ content decreased to 5% of the wild type enzyme, very low activity Klebsiella pneumoniae
7.2.4.2 H76A gamma subunit, almost no effect on activity Klebsiella pneumoniae
7.2.4.2 H77A gamma subunit, Zn2+ content decreased to 10% of the wild type enzyme, very low activity Klebsiella pneumoniae
7.2.4.2 H78A gamma subunit, binding to alpha subunit is lost Klebsiella pneumoniae
7.2.4.2 H78A gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.2 membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.4.2 Zn2+ binds to the gamma subunit, can not be removed with EDTA or 1,10-phenanthroline Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.2 Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.2 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.4.2 recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.2 Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
7.2.4.2 Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.2 trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.2 biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae