Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhuang, Z.; Song, F.; Zhang, W.; Taylor, K.; Archambault, A.; Dunaway-Mariano, D.; Dong, J.; Carey, P.R.
    Kinetic, Raman, NMR, and site-directed mutagenesis studies of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-CoA thioesterase active site (2002), Biochemistry, 41, 11152-11160.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.23 D17E site-directed mutagensis, increased activity Pseudomonas sp.
3.1.2.23 D17N site-directed mutagensis, highly reduced activity Pseudomonas sp.
3.1.2.23 D17S site-directed mutagensis, highly reduced activity Pseudomonas sp.
3.1.2.23 K90A site-directed mutagensis, enhanced sensitivity against inhibitor 4-hydroxyphenacyl-CoA Pseudomonas sp.
3.1.2.23 R126L site-directed mutagensis, slightly reduced activity Pseudomonas sp.
3.1.2.23 R128A site-directed mutagensis, similar to the wild-type enzyme Pseudomonas sp.
3.1.2.23 R88A site-directed mutagensis, slightly reduced activity Pseudomonas sp.
3.1.2.23 R89L site-directed mutagensis, similar to the wild-type enzyme Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.23 4-hydroxyphenacyl-CoA
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.23 0.00014
-
 4-hydroxybenzoyl-CoA mutant K90A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0024
-
 4-hydroxybenzoyl-CoA mutant D17E, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0045
-
 4-hydroxybenzoyl-CoA mutant R88A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.005
-
 4-hydroxybenzoyl-CoA mutant R126L, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0052
-
 4-hydroxybenzoyl-CoA mutant R89L, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.006
-
 4-hydroxybenzoyl-CoA wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0073
-
 4-hydroxybenzoyl-CoA mutant R128A, pH 7.5, 25°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O Pseudomonas sp.
-
 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O Pseudomonas sp. CBS3
-
 4-Hydroxybenzoate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.23 Pseudomonas sp.
-
-
-
3.1.2.23 Pseudomonas sp. CBS3
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA catalytic sites of wild-type and mutant enzymes, substrate binding modifications, reaction kinetics Pseudomonas sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS3 4-Hydroxybenzoate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.23 4-HBA-CoA thioesterase
-
 Pseudomonas sp.
3.1.2.23 4-hydroxybenzoyl coenzyme A hydrolase
-
 Pseudomonas sp.
3.1.2.23 4-hydroxybenzoyl coenzyme A thioesterase
-
 Pseudomonas sp.
3.1.2.23 Hydrolase, 4-hydroxybenzoyl coenzyme A
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.23 0.00055
-
 4-hydroxybenzoyl-CoA mutant D17N, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.00084
-
 4-hydroxybenzoyl-CoA mutant D17S, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.11
-
 4-hydroxybenzoyl-CoA mutant R126L, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.13
-
 4-hydroxybenzoyl-CoA mutant R88A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.14
-
 4-hydroxybenzoyl-CoA mutant K90A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.15
-
 4-hydroxybenzoyl-CoA mutant R128A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.18
-
 4-hydroxybenzoyl-CoA wild-type enzyme and mutant R89L, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.48
-
 4-hydroxybenzoyl-CoA mutant D17E, pH 7.5, 25°C Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.23 0.0011
-
 4-hydroxyphenacyl-CoA mutant R126L, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0012
-
 4-hydroxyphenacyl-CoA mutant R88A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0014
-
 4-hydroxyphenacyl-CoA wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0018
-
 4-hydroxyphenacyl-CoA mutant D17E, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.0021
-
 4-hydroxyphenacyl-CoA mutant R128A, pH 7.5, 25°C Pseudomonas sp.
3.1.2.23 0.004
-
 4-hydroxyphenacyl-CoA mutant K90A, pH 7.5, 25°C Pseudomonas sp.