Literature summary extracted from
Marks, G.T.; Harris, T.K.; Massiah, M.A.; Mildvan, A.S.; Harrison, D.H.
Mechanistic implications of methylglyoxal synthase complexed with phosphoglycolohydroxamic acid as observed by X-ray crystallography and NMR spectroscopy (2001), Biochemistry, 40, 6805-6818.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.3.3 |
sitting drop vapor diffusion method |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.2.3.3 |
D71N |
2500fold decrease in kcat |
Escherichia coli |
General Stability
EC Number |
General Stability |
Organism |
---|
4.2.3.3 |
D71N mutant enzyme rapidly becomes inactive in the absence of phosphate |
Escherichia coli |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.3.3 |
phosphoglycolohydroxamic acid |
tight binding inhibitor, neither a strictly competitive, non-competitive, nor uncompetitive mechanism |
Escherichia coli |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.3.3 |
Escherichia coli |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.2.3.3 |
glycerone phosphate = 2-oxopropanal + phosphate |
mechanism |
Escherichia coli |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.3.3 |
dihydroxyacetone phosphate |
converts dihydrocyacetone phosphate to a cis-enediolic intermediate, then catalyses the elimination of phosphate to form the enol of methylglyoxal |
Escherichia coli |
methylglyoxal + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.3.3 |
hexamer |
homohexamer, crystallization experiments |
Escherichia coli |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
4.2.3.3 |
0.000093 |
- |
phosphoglycolohydroxamic acid |
- |
Escherichia coli |
|