EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.23 | ura3 gene, wild-type and mutant ODCase, expression in Escherichia coli SS6130 | Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.23 | complexed with the inhibitor 6-hydroxyuridine 5-phosphate | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.23 | D37A | 300fold reduced kcat/Km value | Saccharomyces cerevisiae |
4.1.1.23 | T100A | 60fold reduced kcat/Km value | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | 2'-deoxyuridine 5'-phosphate | competitive inhibition, at higher concentrations | Saccharomyces cerevisiae | |
4.1.1.23 | 6-azauridine 5'-phosphate | competitive inhibition | Saccharomyces cerevisiae | |
4.1.1.23 | 6-hydroxyuridine 5'-phosphate | mode of binding | Saccharomyces cerevisiae | |
4.1.1.23 | D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | |
4.1.1.23 | poly(ADP-D-ribose)n-1 | - |
Saccharomyces cerevisiae | |
4.1.1.23 | ribose | weak | Saccharomyces cerevisiae | |
4.1.1.23 | UMP | competitive inhibition | Saccharomyces cerevisiae | |
4.1.1.23 | xanthosine 5'-phosphate | competitive inhibition | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | 0.0007 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0042 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0052 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0054 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0067 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.01 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, D37A mutant | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.23 | soluble | recombinant mutant ODCase | Saccharomyces cerevisiae | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | additional information | functions without metals | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.23 | Orotidine 5'-phosphate | Saccharomyces cerevisiae | catalyzes the final step of pyrimidine biosynthesis | UMP + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.23 | Saccharomyces cerevisiae | P03962 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.23 | - |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.23 | 2'-deoxyorotidine 5'-phosphate | less effective than orotidine 5-phosphate | Saccharomyces cerevisiae | 2'-deoxyuridine 5'-phosphate + CO2 | - |
? | |
4.1.1.23 | orotic acid | extremely poor substrate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.23 | Orotidine 5'-phosphate | enzyme/active site structure, mode of substrate binding | Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
4.1.1.23 | Orotidine 5'-phosphate | catalyzes the final step of pyrimidine biosynthesis | Saccharomyces cerevisiae | UMP + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.23 | dimer | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.23 | 25 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | 0.0017 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.15 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.16 | - |
2'-deoxyorotidine 5'-phosphate | pH 7.2, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.9 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 7.2 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 44 | - |
orotidine 5'-phosphate | pH 7.2, 25°C, wild-type enzyme | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.23 | 7.2 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | additional information | functions without cofactors | Saccharomyces cerevisiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | 0.000064 | - |
6-azauridine 5'-phosphate | pH 7.4, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.00041 | - |
xanthosine 5'-phosphate | pH 7.4, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0077 | - |
6-azauridine 5'-phosphate | pH 7.4, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.0094 | - |
6-azauridine 5'-phosphate | pH 7.4, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.012 | - |
xanthosine 5'-phosphate | pH 7.4, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.039 | - |
xanthosine 5'-phosphate | pH 7.4, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.081 | - |
D-ribose 5-phosphate | pH 7.4, 25°C | Saccharomyces cerevisiae | |
4.1.1.23 | 0.2 | - |
UMP | pH 7.4, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.21 | - |
UMP | pH 7.4, 25°C, D37A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.26 | - |
2'-deoxyuridine 5'-phosphate | pH 7.4, 25°C | Saccharomyces cerevisiae | |
4.1.1.23 | 0.37 | - |
UMP | pH 7.4, 25°C, T100A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.7 | - |
poly(ADP-D-ribose)n-1 | pH 7.4, 25°C | Saccharomyces cerevisiae | |
4.1.1.23 | 37 | - |
ribose | pH 7.4, 25°C | Saccharomyces cerevisiae |