Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miller, B.G.; Butterfoss, G.L.; Short, S.A.; Wolfenden, R.
    Role of enzyme-ribofuranosyl contacts in the ground state and transition state for orotidine 5'-phosphate decarboxylase: a role for substrate destabilization? (2001), Biochemistry, 40, 6227-6232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.23 ura3 gene, wild-type and mutant ODCase, expression in Escherichia coli SS6130 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.23 complexed with the inhibitor 6-hydroxyuridine 5’-phosphate Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.23 D37A 300fold reduced kcat/Km value Saccharomyces cerevisiae
4.1.1.23 T100A 60fold reduced kcat/Km value Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.23 2'-deoxyuridine 5'-phosphate competitive inhibition, at higher concentrations Saccharomyces cerevisiae
4.1.1.23 6-azauridine 5'-phosphate competitive inhibition Saccharomyces cerevisiae
4.1.1.23 6-hydroxyuridine 5'-phosphate mode of binding Saccharomyces cerevisiae
4.1.1.23 D-ribose 5-phosphate
-
Saccharomyces cerevisiae
4.1.1.23 poly(ADP-D-ribose)n-1
-
Saccharomyces cerevisiae
4.1.1.23 ribose weak Saccharomyces cerevisiae
4.1.1.23 UMP competitive inhibition Saccharomyces cerevisiae
4.1.1.23 xanthosine 5'-phosphate competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.23 0.0007
-
orotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.0042
-
orotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 0.0052
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.0054
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.0067
-
orotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.01
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.23 soluble recombinant mutant ODCase Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.23 additional information functions without metals Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.23 Orotidine 5'-phosphate Saccharomyces cerevisiae catalyzes the final step of pyrimidine biosynthesis UMP + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.23 Saccharomyces cerevisiae P03962
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.23
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.23 2'-deoxyorotidine 5'-phosphate less effective than orotidine 5’-phosphate Saccharomyces cerevisiae 2'-deoxyuridine 5'-phosphate + CO2
-
?
4.1.1.23 orotic acid extremely poor substrate Saccharomyces cerevisiae ?
-
?
4.1.1.23 Orotidine 5'-phosphate enzyme/active site structure, mode of substrate binding Saccharomyces cerevisiae UMP + CO2
-
?
4.1.1.23 Orotidine 5'-phosphate catalyzes the final step of pyrimidine biosynthesis Saccharomyces cerevisiae UMP + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.23 dimer
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.23 25
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.23 0.0017
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 0.15
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.16
-
2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.9
-
orotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 7.2
-
orotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 44
-
orotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.23 7.2
-
assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.23 additional information functions without cofactors Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.23 0.000064
-
6-azauridine 5'-phosphate pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.00041
-
xanthosine 5'-phosphate pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.0077
-
6-azauridine 5'-phosphate pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 0.0094
-
6-azauridine 5'-phosphate pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.012
-
xanthosine 5'-phosphate pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.039
-
xanthosine 5'-phosphate pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 0.081
-
D-ribose 5-phosphate pH 7.4, 25°C Saccharomyces cerevisiae
4.1.1.23 0.2
-
UMP pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.21
-
UMP pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
4.1.1.23 0.26
-
2'-deoxyuridine 5'-phosphate pH 7.4, 25°C Saccharomyces cerevisiae
4.1.1.23 0.37
-
UMP pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
4.1.1.23 0.7
-
poly(ADP-D-ribose)n-1 pH 7.4, 25°C Saccharomyces cerevisiae
4.1.1.23 37
-
ribose pH 7.4, 25°C Saccharomyces cerevisiae