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Literature summary extracted from

  • Johnson, K.A.; Chen, L.; Yang, H.; Roberts, M.F.; Stec, B.
    Crystal structure and catalytic mechanism of the MJ0109 gene product: A bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities (2001), Biochemistry, 40, 618-630.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.25 hanging drop vapor diffusion method, in presence of Ca2+, Zn2+ and substrate or product Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Ca2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Li+ poor inhibition Methanocaldococcus jannaschii
3.1.3.25 Zn2+
-
 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Ca2+ two ions bound per subunit Methanocaldococcus jannaschii
3.1.3.25 Mn2+ three ions bound per subunit Methanocaldococcus jannaschii
3.1.3.25 Zn2+ three ions bound per subunit Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Methanocaldococcus jannaschii Q57573 strain MJ109
-
3.1.3.25 Methanocaldococcus jannaschii DSM 2661 Q57573 strain MJ109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 recombinant protein from Escherichia coli Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O 600% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
3.1.3.25 2'-AMP + H2O 600% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii DSM 2661 adenosine + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Methanocaldococcus jannaschii 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Methanocaldococcus jannaschii DSM 2661 4-nitrophenol + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii alpha-D-glucose + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii DSM 2661 alpha-D-glucose + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 2,6-bisphosphate + H2O 16% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii glycerol + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii DSM 2661 glycerol + phosphate
-
 ?
3.1.3.25 additional information D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed Methanocaldococcus jannaschii ?
-
 ?
3.1.3.25 additional information D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 2-phosphate + H2O 15% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer crystal structure analysis Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 More archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Methanocaldococcus jannaschii