EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.15 | recombinant His-tagged wild-type and mutants from overexpressing Escherichia coli BL21 | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.15 | E103A | unaltered thermostability, no remaining prolylation activity, 5% remaining cysteinylation activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
6.1.1.15 | P100A | unaltered thermostability, loss of 90% cysteinylation activity, unaltered prolylation activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.15 | L-cysteine | competitive inhibition of prolylation. A 40fold excess over L-proline concentration reduces the prolylation activity by 80%, no inhibition of mutant P100A | Methanocaldococcus jannaschii | |
6.1.1.15 | L-proline | competitive inhibition of cysteinylation. A 40fold excess over L-cysteine concentration reduces the cysteinylation activity by over 80%, no inhibition of mutant E103A | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.022 | - |
L-cysteine | aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
6.1.1.15 | 0.027 | - |
L-proline | aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
6.1.1.15 | 0.049 | - |
ATP | aminoacylation reaction with L-proline, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
6.1.1.15 | 0.06 | - |
ATP | aminoacylation reaction with L-cysteine, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii DSM 2661 | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.15 | Methanocaldococcus jannaschii | Q58635 | - |
- |
6.1.1.15 | Methanocaldococcus jannaschii DSM 2661 | Q58635 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-cysteine + tRNA | in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity | Methanocaldococcus jannaschii | AMP + diphosphate + L-cysteinyl-tRNA | - |
? | |
6.1.1.15 | ATP + L-cysteine + tRNA | in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-cysteinyl-tRNA | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.15 | ProCysRS | misleading | Methanocaldococcus jannaschii |
6.1.1.15 | proline/cysteine-tRNA ligase | misleading | Methanocaldococcus jannaschii |
6.1.1.15 | prolyl-cysteinyl-tRNA synthetase | misleading | Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 70 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.02 | - |
L-cysteine | aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
6.1.1.15 | 0.09 | - |
L-proline | aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 7 | - |
assay at | Methanocaldococcus jannaschii |