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Literature summary extracted from
- Glutamate 47 in 1-aminocyclopropane-1-carboxylate synthase is a major specificity determinant (2001), Biochemistry, 40, 12276-12284.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.4.1.14 | expression of ACC synthase in Escherichia coli | Malus domestica |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.4.1.14 | E47D | 3.8% of wild-type ACC synthase activity | Malus domestica |
| 4.4.1.14 | E47Q | 0.9% of wild-type ACC synthase activity | Malus domestica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.14 | L-Vinylglycine | mechanism-based inactivation | Malus domestica |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.14 | 0.026 | - |
(S,S)-S-adenosylmethionine | alpha,gamma-elimination | Malus domestica | |
| 4.4.1.14 | 0.037 | - |
(R,S)-S-adenosylmethionine | beta,gamma-elimination | Malus domestica | |
| 4.4.1.14 | 0.27 | - |
L-Vinylglycine | deamination | Malus domestica | |
| 4.4.1.14 | 37 | - |
alanine | transamination | Malus domestica | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.14 | S-adenosyl-L-methionine | Malus domestica | - |
1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.14 | Malus domestica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.14 | recombinant ACC synthase | Malus domestica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.14 | (R,S)-S-adenosyl-L-methionine | - |
Malus domestica | vinylglycine + methylthioadenosine | - |
? | |
| 4.4.1.14 | (S,S)-S-adenosyl-L-methionine | - |
Malus domestica | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
| 4.4.1.14 | L-vinylglycine | - |
Malus domestica | alpha-ketobutyrate + ammonia | - |
? | |
| 4.4.1.14 | pyridoxal 5'-phosphate + alanine | - |
Malus domestica | pyridoxamine 5'-phosphate + pyruvate | - |
? | |
| 4.4.1.14 | S-adenosyl-L-methionine | - |
Malus domestica | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.14 | ACC synthase | - |
Malus domestica |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.14 | 0.019 | - |
alanine | transamination | Malus domestica | |
| 4.4.1.14 | 0.079 | - |
(R,S)-S-adenosylmethionine | beta,gamma-elimination | Malus domestica | |
| 4.4.1.14 | 0.7 | 1 | L-Vinylglycine | deamination | Malus domestica | |
| 4.4.1.14 | 0.71 | - |
L-Vinylglycine | deamination | Malus domestica | |
| 4.4.1.14 | 18 | - |
(S,S)-S-adenosylmethionine | alpha,gamma-elimination | Malus domestica | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.14 | pyridoxal 5'-phosphate | - |
Malus domestica | |
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Release 2023.1 (January 2023)
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