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Literature summary extracted from

  • Boehlein, S.K.; Nakatsu, T.; Hiratake, J.; Thirumoorty, R.; Stewart, J.D.; Richards, N.G.J.; Schuster, S.M.
    Characterization of inhibitors acting at the synthetase site of Escherichia coli asparagine synthetase B (2001), Biochemistry, 40, 11168-11175.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 overexpression in Escherichia col Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.1 adenylated sulfoximine
-
 Escherichia coli
6.3.5.4 adenylated sulfoximine 0.005 mM, 65% inhibition, dead-end complex with AS-B Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.1 ATP + L-aspartate + NH3 Escherichia coli
-
 AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 ATP + L-aspartate + L-glutamine Escherichia coli
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 Escherichia coli asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine Escherichia coli
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.1 Escherichia coli
-
-
-
6.3.5.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.1 ATP + L-aspartate + NH3
-
 Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 ATP + cysteine sulfinic acid
-
 Escherichia coli AMP + diphosphate + cysteine sulfinic acid
-
 ?
6.3.5.4 ATP + L-aspartate + L-glutamine
-
 Escherichia coli AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.1 AS-A
-
 Escherichia coli
6.3.1.1 Asparagine synthetase A
-
 Escherichia coli
6.3.5.4 AS-B
-
 Escherichia coli
6.3.5.4 Asparagine synthetase B
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.1.1 0.000067
-
 adenylated sulfoximine
-
 Escherichia coli