Any feedback?
Literature summary extracted from
- Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-CoA mutase (2000), Biochemistry, 39, 9213-9221.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | expression in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | hanging drop method | Propionibacterium freudenreichii subsp. shermanii |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | H224Q | lower turnover than wild-type enzyme | Propionibacterium freudenreichii subsp. shermanii |
| 5.4.99.2 | H244A | lower turnover than wild-type enzyme | Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 0.083 | - |
succinyl-CoA | 25°C, mutant H244A | Propionibacterium freudenreichii subsp. shermanii |  |
| 5.4.99.2 | 0.085 | - |
succinyl-CoA | 25°C, mutant H244Q | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 0.096 | - |
succinyl-CoA | 25°C, wild-type | Propionibacterium freudenreichii subsp. shermanii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | Propionibacterium freudenreichii subsp. shermanii | - |
succinyl-CoA | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | - |
Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | succinyl-CoA | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | methylmalonyl-CoA mutase | - |
Propionibacterium freudenreichii subsp. shermanii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 0.038 | - |
succinyl-CoA | 25°C, mutant H244A | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 0.66 | - |
succinyl-CoA | 25°C, mutant H244Q | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 6.08 | - |
succinyl-CoA | 25°C, mutant H244Q | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 48 | - |
succinyl-CoA | 25°C, wild-type | Propionibacterium freudenreichii subsp. shermanii | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | adenosylcobalamin | - |
Propionibacterium freudenreichii subsp. shermanii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
