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Literature summary extracted from
- Effects of Mg2+ on the pre-steady-state kinetics of the biotin carboxylation reaction of pyruvate carboxylase (2000), Biochemistry, 39, 7480-7491.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 6.4.1.1 | dilution inactivates the enzyme, inactivation can be prevented at 10 mM Mg2+ | Gallus gallus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.1 | Mg2+ | 10 mM | Gallus gallus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.1 | ATP + pyruvate + HCO3- | Gallus gallus | - |
ADP + phosphate + oxaloacetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.4.1.1 | Gallus gallus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.4.1.1 | - |
Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.4.1.1 | liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.1 | 2030 | - |
- |
Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.1 | ATP + pyruvate + HCO3- | - |
Gallus gallus | ADP + phosphate + oxaloacetate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.4.1.1 | 0.0018 | - |
ATP | pH 7.8, 30°C, absence of acetyl CoA, 0.24 mM Mg2+ | Gallus gallus | |
| 6.4.1.1 | 0.0029 | - |
ATP | pH 7.8, 30°C, absence of acetyl CoA, 10 mM Mg2+ | Gallus gallus | |
| 6.4.1.1 | 0.0049 | - |
ATP | pH 7.8, 30°C, absence of acetyl CoA, 1 mM Mg2+ | Gallus gallus | |
| 6.4.1.1 | 0.054 | - |
ATP | pH 7.8, 30°C, presence of acetyl CoA, 1 mM Mg2+ | Gallus gallus | |
| 6.4.1.1 | 0.28 | - |
ATP | pH 7.8, 30°C, presence of acetyl CoA, 0.24 mM Mg2+ | Gallus gallus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.1 | biotin | dependent, enzyme contains biotin | Gallus gallus | |
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