Literature summary extracted from

Stanley, T.M.; Johnson, W.H., Jr.; Burks, E.A.; Whitman, C.P.; Hwang, C.C.; Cook, P.F.
Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase (2000), Biochemistry, 39, 718-726.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.1.77	-	Pseudomonas putida

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.1.77	68	-	2-oxo-3-hexenedioate	pH 7.3	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.77	Pseudomonas putida	-	mt-2	-
4.1.1.77	Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182	-	mt-2	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.1.77	complex with vinylpyruvate hydratase, homogenetiy	Pseudomonas putida

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
4.1.1.77	(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2	mechanism	Pseudomonas putida	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.77	2-oxo-3-hexenedioate	4-OD and vinylpyruvate hydratase form a complex, 2-hydroxy-2,4-pentadienoate is the product of 4-OD and 2-oxo-4-pentenoate results from a vinylpyruvate hydratase-catalyzed process	Pseudomonas putida	2-oxopent-4-enoate + CO2	-	?
4.1.1.77	2-oxo-3-hexenedioate	4-OD and vinylpyruvate hydratase form a complex, 2-hydroxy-2,4-pentadienoate is the product of 4-OD and 2-oxo-4-pentenoate results from a vinylpyruvate hydratase-catalyzed process	Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182	2-oxopent-4-enoate + CO2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.1.77	4-OD	-	Pseudomonas putida

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.1.1.77	68	-	2-oxo-3-hexenedioate	-	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)