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Literature summary extracted from

  • Chan, E.C.; Chang, C.C.; Li, Y.S.; Chang, C.A.; Chiou, C.C.; Wu, T.Z.
    Purification and characterization of neutral sphingomyelinase from Helicobacter pylori (2000), Biochemistry, 39, 4838-4845.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 EDTA 20 mM, complete inhibition Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ required for activity, maximal activity at 8-16 mM, Km at 3 mM Helicobacter pylori
3.1.4.12 Mn2+ can replace Mg2+, Km at 2.5 mM Helicobacter pylori
3.1.4.12 Zn2+ slight activation at 4 mM, Km at 2 mM Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 phosphatidylethanolamine + H2O Helicobacter pylori low activity diacylglycerol + ethanolamine phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O Helicobacter pylori
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 acid glycine, ammonium sulfate, CM Sepharose, Mono Q, Sephadex G-75 Helicobacter pylori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 0.014
-
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 phosphatidylethanolamine + H2O low activity Helicobacter pylori diacylglycerol + ethanolamine phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Helicobacter pylori N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 N-SMase
-
 Helicobacter pylori
3.1.4.12 neutral sphingomyelinase
-
 Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7.4
-
-
 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.12 6 8.5 approx. 10% of maximal activity at pH 6.0 and pH 8.5, respectively Helicobacter pylori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.12 Helicobacter pylori isoelectric focusing
-
 7.15