BRENDA - Enzyme Database

Purification and characterization of neutral sphingomyelinase from Helicobacter pylori

Chan, E.C.; Chang, C.C.; Li, Y.S.; Chang, C.A.; Chiou, C.C.; Wu, T.Z.; Biochemistry 39, 4838-4845 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
EDTA
20 mM, complete inhibition
Helicobacter pylori
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
required for activity, maximal activity at 8-16 mM, Km at 3 mM
Helicobacter pylori
3.1.4.12
Mn2+
can replace Mg2+, Km at 2.5 mM
Helicobacter pylori
3.1.4.12
Zn2+
slight activation at 4 mM, Km at 2 mM
Helicobacter pylori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
phosphatidylethanolamine + H2O
Helicobacter pylori
low activity
diacylglycerol + ethanolamine phosphate
-
Helicobacter pylori
?
3.1.4.12
sphingomyelin + H2O
Helicobacter pylori
-
N-acylsphingosine + choline phosphate
-
Helicobacter pylori
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Helicobacter pylori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.12
acid glycine, ammonium sulfate, CM Sepharose, Mono Q, Sephadex G-75
Helicobacter pylori
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.12
0.014
-
-
Helicobacter pylori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
phosphatidylethanolamine + H2O
low activity
649984
Helicobacter pylori
diacylglycerol + ethanolamine phosphate
-
649984
Helicobacter pylori
?
3.1.4.12
sphingomyelin + H2O
-
649984
Helicobacter pylori
N-acylsphingosine + choline phosphate
-
649984
Helicobacter pylori
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7.4
-
-
Helicobacter pylori
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.12
6
8.5
approx. 10% of maximal activity at pH 6.0 and pH 8.5, respectively
Helicobacter pylori
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.4.12
Helicobacter pylori
isoelectric focusing
-
7.15
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
EDTA
20 mM, complete inhibition
Helicobacter pylori
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
required for activity, maximal activity at 8-16 mM, Km at 3 mM
Helicobacter pylori
3.1.4.12
Mn2+
can replace Mg2+, Km at 2.5 mM
Helicobacter pylori
3.1.4.12
Zn2+
slight activation at 4 mM, Km at 2 mM
Helicobacter pylori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
phosphatidylethanolamine + H2O
Helicobacter pylori
low activity
diacylglycerol + ethanolamine phosphate
-
Helicobacter pylori
?
3.1.4.12
sphingomyelin + H2O
Helicobacter pylori
-
N-acylsphingosine + choline phosphate
-
Helicobacter pylori
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.12
acid glycine, ammonium sulfate, CM Sepharose, Mono Q, Sephadex G-75
Helicobacter pylori
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.12
0.014
-
-
Helicobacter pylori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
phosphatidylethanolamine + H2O
low activity
649984
Helicobacter pylori
diacylglycerol + ethanolamine phosphate
-
649984
Helicobacter pylori
?
3.1.4.12
sphingomyelin + H2O
-
649984
Helicobacter pylori
N-acylsphingosine + choline phosphate
-
649984
Helicobacter pylori
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7.4
-
-
Helicobacter pylori
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.12
6
8.5
approx. 10% of maximal activity at pH 6.0 and pH 8.5, respectively
Helicobacter pylori
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.4.12
Helicobacter pylori
isoelectric focusing
-
7.15