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Literature summary extracted from

  • Jockel, P.; Schmid, M.; Choinowski, T.; Dimroth, P.
    Essential role of tyrosine 229 of the oxaloacetate decarboxylase beta-subunit in the energy coupling mechanism of the Na(+) pump (2000), Biochemistry, 39, 4320-4326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.2 expressed in Escherichia coli DH5alpha/pSK-GAB Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
7.2.4.2 C291E beta subunit, unstable in the absence of Na+, dissociates into an alpha-gamma subcomplex and the beta subunit Klebsiella pneumoniae
7.2.4.2 D203N beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 G337A beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 G377A beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 G380A beta subunit, about 30% of activity Klebsiella pneumoniae
7.2.4.2 N373L beta subunit, almost complete loss of activity Klebsiella pneumoniae
7.2.4.2 Q192L beta subunit, lower pH optimum than wild type enzyme Klebsiella pneumoniae
7.2.4.2 R389A beta subunit, about 10% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
7.2.4.2 R389D beta subunit, almost complete loss of activity Klebsiella pneumoniae
7.2.4.2 R389K beta subunit, about 80% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
7.2.4.2 R389L beta subunit, about 10% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
7.2.4.2 S382A beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 S382C beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 S382D beta subunit, about 10% of activity Klebsiella pneumoniae
7.2.4.2 S382E beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 S382N beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 S382Q beta subunit, complete loss of activity Klebsiella pneumoniae
7.2.4.2 S382T beta subunit, about 10% of activity Klebsiella pneumoniae
7.2.4.2 Y209A beta subunit, lower pH optimum than wild type enzyme Klebsiella pneumoniae
7.2.4.2 Y229F beta subunit, completely inactive Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.2 membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.4.2 Na+ two ions per enzyme required for maximum activity, cooperative binding, 20-50 mM protects against tryptic digestion Klebsiella pneumoniae
7.2.4.2 Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.2 Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.2 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.4.2 recombinant protein from Escherichia coli Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.4.2 additional information
-
 specific activity for all mutant enzymes Klebsiella pneumoniae
7.2.4.2 45
-
 wild type enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.2 Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
7.2.4.2 Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.2 trimer alpha, beta, gamma Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.4.2 5.5
-
 Q192L and Y209A mutants Klebsiella pneumoniae
7.2.4.2 6.5 7.5 wild type enzyme Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.2 biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae