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Literature summary extracted from

  • Martin, S.F.; Follows, B.C.; Hergenrother, P.J.; Trotter, B.K.
    The choline binding site of phospholipase C (Bacillus cereus): insights into substrate specificity (2000), Biochemistry, 39, 3410-3415.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 Zn2+ required, three ions per active site Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 E4D significant decrease in catalytic efficiency Bacillus cereus
3.1.4.3 E4L significant decrease in catalytic efficiency Bacillus cereus
3.1.4.3 E4Q significant decrease in catalytic efficiency Bacillus cereus
3.1.4.3 F66A dramatic decrease in catalytic efficiency Bacillus cereus
3.1.4.3 F66R dramatic decrease in catalytic efficiency Bacillus cereus
3.1.4.3 F66W catalytic activity comparable to wild type Bacillus cereus
3.1.4.3 Y56A little decrease in catalytic efficiency Bacillus cereus
3.1.4.3 Y56R little decrease in catalytic efficiency Bacillus cereus
3.1.4.3 Y56W little decrease in catalytic efficiency Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 1.8
-
 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine pH 7.3 Bacillus cereus
3.1.4.3 2.4
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine pH 7.3 Bacillus cereus
3.1.4.3 4.5
-
 1,2-dihexanoyl-sn-glycero-3-phospho-L-serine pH 7.3 Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 28500
-
 x * 28500, deduced from gene sequence Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus P09598
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dihexanoyl-sn-glycero-3-phospho-L-serine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphorylserine
-
 ?
3.1.4.3 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphorylcholine
-
 ?
3.1.4.3 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 ? x * 28500, deduced from gene sequence Bacillus cereus