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Literature summary extracted from

  • Huang, X.; Raushel, F.M.
    An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammonia (2000), Biochemistry, 39, 3240-3247.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 A309C kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 A309C/S35C kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 A309S kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 G293A kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 G293I kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 G293S kinetic properties are similar to those of the wild-type enzyme Escherichia coli
6.3.5.5 G359F glutaminase and bicarbonate-dependent ATPase reaction are uncoupled from one another, the mutant enzyme is fully functional when external ammonia is utilized as the nitrogen source but is unable to use glutamine for the synthesis of carbamoyl phosphate Escherichia coli
6.3.5.5 G359Y glutaminase and bicarbonate-dependent ATPase reaction are uncoupled from one another, the mutant enzyme is fully functional when external ammonia is utilized as the nitrogen source but is unable to use glutamine for the synthesis of carbamoyl phosphate Escherichia coli
6.3.5.5 S35C kinetic properties are similar to those of the wild-type enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 42000
-
 1 * 42000 + 1 * 118000 Escherichia coli
6.3.5.5 118000
-
 1 * 42000 + 1 * 118000 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.5.5 additional information existence of an alternate route to the bicarbonate phosphorylation site when ammonia is provided as an external nitrogen source Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 heterodimer 1 * 42000 + 1 * 118000 Escherichia coli