Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cottrell, G.S.; Hyde, R.J.; Lim, J.; Parsons, M.R.; Hooper, N.M.; Turner, A.J.
    Identification of critical residues in the active site of porcine membrane-bound aminopeptidase P (2000), Biochemistry, 39, 15129-15135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9 transient expression of wild-type and mutants in COS-1 cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.9 D449A site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 D449N site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 D460A site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 D460N site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 E554A site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 E554Q site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 E568A site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 E568Q site-directed mutagenesis, low expression level in COS-1 cells, inactive mutant Sus scrofa
3.4.11.9 E588A site-directed mutagenesis, 66% remaining activity compared to the wild-type enzyme with substrate bradykinin Sus scrofa
3.4.11.9 E588Q site-directed mutagenesis, 83% remaining activity compared to the wild-type enzyme with substrate bradykinin Sus scrofa
3.4.11.9 H429K site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 H429L site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 H519K site-directed mutagenesis, 2.3% remaining activity compared to the wild-type enzyme with substrate bradykinin, increased Km, reduced kcat Sus scrofa
3.4.11.9 H519L site-directed mutagenesis, 73.8% remaining activity compared to the wild-type enzyme with substrate bradykinin, increased Km Sus scrofa
3.4.11.9 H523K site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 H523L site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 H532K site-directed mutagenesis, inactive mutant Sus scrofa
3.4.11.9 H532L site-directed mutagenesis, very low expression level in COS-1 cells, protein degradation, inactive mutant Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.9 0.058
-
 bradykinin 37°C, wild-type enzyme Sus scrofa
3.4.11.9 0.391
-
 bradykinin 37°C, mutant H519L Sus scrofa
3.4.11.9 0.5
-
 bradykinin 37°C, mutant H519K Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.9 membrane bound Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Zn2+ mono-zinc-containing enzyme, lacks any of the typical metal binding motifs found in other zinc metalloproteases Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 91000
-
 x * 91000, wild-type enzyme, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.9 glycoprotein
-
 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.9 release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide acive site configuration, modeling, Asp449, Asp460, His523, Glu554, and Glu568 are in volved in metal binding in the active site, His429 and His523 are involved in shuttling protons during catalysis Sus scrofa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.9 0.0375
-
 recombinant wild-type enzyme, substrate bradykinin Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 bradykinin + H2O hydrolysis of the N-terminal Arg1-Pro2 bond Sus scrofa Arg + des-Arg-bradykinin
-
 ?
3.4.11.9 Gly-Pro-hydroxyPro + H2O
-
 Sus scrofa Gly + Pro-hydroxyPro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 ? x * 91000, wild-type enzyme, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.11.9 aminopeptidase P
-
 Sus scrofa
3.4.11.9 AP-P
-
 Sus scrofa
3.4.11.9 More the enzyme belongs to the peptidase family M24 Sus scrofa
3.4.11.9 X-Pro aminopeptidase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.9 37
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.9 0.17
-
 bradykinin 37°C, mutant H519K Sus scrofa
3.4.11.9 1.18
-
 bradykinin 37°C, wild-type enzyme Sus scrofa
3.4.11.9 1.82
-
 bradykinin 37°C, mutant H519L Sus scrofa
3.4.11.9 2.94
-
 bradykinin 37°C, mutant H519L Sus scrofa
3.4.11.9 6.08
-
 bradykinin 37°C, wild-type enzyme Sus scrofa