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Literature summary extracted from

  • Radfar, R.; Leaphart, A.; Brewer, J.M.; Minor, W.; Odom, J.D.; Dunlap, R.B.; Lovell, C.R.; Lebioda, L.
    Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica (2000), Biochemistry, 39, 14481-14486.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3 expression in Escherichia coli Moorella thermoacetica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.3 crystals of FTHFS complexed with NH4+, K+ or Cs+ are grown by vapor diffusion from 46% saturated ammonium sulfate, 1 mM dithiothreitol and 1% polyehtylene glycol 1000 in 50 mM potassium maleate buffer, pH 7.6, crystals diffract to 3.0-3.2 A resolution Moorella thermoacetica

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 E98D 36% of wild-type activity Moorella thermoacetica
6.3.4.3 E98Q 93% of wild-type activity, slight increase in thermal stability in the absence of monovalent cation Moorella thermoacetica
6.3.4.3 E98S 61% of wild-type activity Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.3 K+ monovalent cation required for activity Moorella thermoacetica
6.3.4.3 NH4+ monovalent cation required for activity Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 60000
-
 4 * 60000, SDS-PAGE Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Moorella thermoacetica
-
 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Moorella thermoacetica P21164
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 recombinant FTHFS Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.3 780
-
 recombinant FTHFS Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.3 tetramer 4 * 60000, SDS-PAGE Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 FTHFS
-
 Moorella thermoacetica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.3 55 60
-
 Moorella thermoacetica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.3 69
-
 in the absence of monovalent cation Moorella thermoacetica
6.3.4.3 79
-
 in the presence of 200 mM K+ Moorella thermoacetica