Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, L.; Zhou, C.; Yang, H.; Roberts, M.F.
    Inositol-1-phosphate synthase from Archaeoglobus fulgidus is a class II aldolase (2000), Biochemistry, 39, 12415-12423.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.4 overexpression in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.4 EDTA 0.002 mM, 50% inhibition Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.4 0.0051
-
 NAD+ 90°C, pH 7.5 Archaeoglobus fulgidus
5.5.1.4 0.12
-
 D-glucose 6-phosphate 90°C, pH 7.5 Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.4 Co2+ absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ Archaeoglobus fulgidus
5.5.1.4 Mg2+ KM: 0.0242 mM, activates Archaeoglobus fulgidus
5.5.1.4 Mn2+ Km: 0.00077 mM, absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ Archaeoglobus fulgidus
5.5.1.4 Zn2+ Km: 0.0011 mM, absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.4 44100
-
 4 * 44100, SDS-PAGE Archaeoglobus fulgidus
5.5.1.4 168000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.4 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.4 recombinant enzyme Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.4 11.8
-
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.4 D-glucose 6-phosphate class II aldolase Archaeoglobus fulgidus 1D-myo-inositol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.4 tetramer 4 * 44100, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
5.5.1.4 inositol-1-phosphate synthase
-
 Archaeoglobus fulgidus
5.5.1.4 IPS
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.4 90
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.5.1.4 76 100 76°C: about 40% of maximal activity, 100°C: about 50% of maximal activity Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.4 9.6
-
 D-glucose 6-phosphate
-
 Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.4 8
-
 at 85°C Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.4 6.5 9.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 95% of maximal activity Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.4 NAD+ required Archaeoglobus fulgidus