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Literature summary extracted from

  • Mueller, E.J.; Oh, S.; Kavalerchik, E.; Kappock, T.J.; Meyer, E.; Li, C.; Ealick, S.E.; Stubbe, J.
    Investigation of the ATP Binding Site of Escherichia coli Aminoimidazole Ribonucleotide Synthetase Using Affinity Labeling and Site-Directed Mutagenesis (1999), Biochemistry, 38, 9831-9839.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.1 expression of recombinant enzymes in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.1 K27L obtained by site-directed mutagenesis and expression in Escherichia coli Escherichia coli
6.3.3.1 K27Q obtained by site-directed mutagenesis and expression in Escherichia coli Escherichia coli
6.3.3.1 K27R obtained by site-directed mutagenesis and expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.1 fluorosulfonylbenzoyl adenosine FSBA, time-dependent inactivation by covalent binding to Lys27 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.1 0.012
-
ATP pH 7.7, K27R mutant Escherichia coli
6.3.3.1 0.046
-
ATP pH 7.7, K27L mutant Escherichia coli
6.3.3.1 0.058
-
ATP pH 7.7, K27Q mutant Escherichia coli
6.3.3.1 0.073
-
ATP pH 7.7, wild type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.1 Escherichia coli P08178
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.1 purification of wild type and recombinant enzymes using C8-linked ATP affinity column Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.1 ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine
-
Escherichia coli ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole
-
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Synonyms

EC Number Synonyms Comment Organism
6.3.3.1 aminoimidazole ribonucleotide synthetase
-
Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.3.1 0.025
-
fluorosulfonylbenzoyl adenosine 15°C, pH 7.7 Escherichia coli