BRENDA - Enzyme Database show

Binding of biliverdin, bilirubin, and thyroid hormones to lipocalin-type prostaglandin D synthase

Beuckmann, C.T.; Aoyagi, M.; Okazaki, I.; Hiroike, T.; Toh, H.; Hayaishi, O.; Urade, Y.; Biochemistry 38, 8006-8013 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expression in Escherichia coli
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
3,3',5'-triiodo-L-thyronine
0.0093 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
3,3',5-triiodo-L-thyronine
0.011 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
bilirubin
0.0068 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
Biliverdin
0.0053 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
L-thyroxine
0.0039 mM, 50% inhibition, noncompetitve inhibition
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS-glutathione transferase fusion protein
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649905
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649905
Rattus norvegicus
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.3.99.2
0.023
-
L-thyroxine
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expression in Escherichia coli
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
3,3',5'-triiodo-L-thyronine
0.0093 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
3,3',5-triiodo-L-thyronine
0.011 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
bilirubin
0.0068 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
Biliverdin
0.0053 mM, 50% inhibition
Rattus norvegicus
5.3.99.2
L-thyroxine
0.0039 mM, 50% inhibition, noncompetitve inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.3.99.2
0.023
-
L-thyroxine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS-glutathione transferase fusion protein
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649905
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649905
Rattus norvegicus
?