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Literature summary extracted from

  • Fan, Y.X.; McPhie, P.; Miles, E.W.
    Guanidine hydrochloride exerts dual effects on the tryptophan synthase alpha2beta2 complex as a cation Activator and as a modulator of the active site conformation (1999), Biochemistry, 38, 7881-7890.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.20 guanidine hydrochloride stimulating, dual effector as cation activator and as a modulator of the active site conformation by alteration of the equilibrium distribution of pyridoxal 5'-phosphate intermediates formed in reaction with the beta-subunit, effects on reaction are highly dependent on the substrate, effects are altered by NaCl Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expression of wild-type enzyme complex and of the beta-subunit dimer in Escherichia coli CB149 Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
4.2.1.20 binding of allosteric ligands stabilizes the enzyme's conformation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.20 Urea the beta-elimination activity of the enzyme complex decrease less sharply than the beta-replacement activity with increasing concentration in absence of NaCl, 0.1 M NaCl alters the effect of urea concentration Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.20 NaCl ligand binding sites, effector on the action of activators or inhibitors, overview Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 recombinant wild-type enzyme complex and of the beta-subunit dimer from Escherichia coli CB149 Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
?
4.2.1.20 beta-chloro-L-alanine in absence of indole Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3 + HCl
-
?
4.2.1.20 beta-chloro-L-alanine + indole
-
Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + HCl
-
?
4.2.1.20 L-serine
-
Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
?
4.2.1.20 additional information reaction intermediates formed in the different reactions, conformational transition states regulate the activity and specificity of the enzyme complex Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 More conformational states, overview, subunit dissociation in presence of guanidinium hydrochloride Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.20 25
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.20 7.8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium