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Literature summary extracted from

  • Bueso, J.; Cervera, J.; Fresquet, V.; Marina, A.; Lusty, C.J.; Rubio, V.
    Photoaffinity labeling with the activator IMP and Site-Directed mutagenesis of histidine 995 of carbamoyl phosphate synthetase from Escherichia coli demonstrate that the binding site for IMP overlaps with that for the inhibitor UMP (1999), Biochemistry, 38, 3910-3917.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 IMP His995, located in the C-terminal regulatory domain, is cross-linked with IMP Escherichia coli
6.3.5.5 ornithine
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 H995A mutant exhibits normal kinetics for the activator ornithine and for the substrates in presence of ornithine, the mutation selectiviely decrease 5.6 and 2.3fold the apparent affinity for UMP and IMP, mutation abolishes the ability to be photoaffinity labeled with IMP Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP Lys993 is cross-linked with UMP Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- Escherichia coli the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?