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Literature summary extracted from

  • Jockel, P.; Di Berardino, M.; Dimroth, P.
    Membrane topology of the beta-subunit of the oxaloacetate decarboxylase Na+ pump from Klebsiella pneumoniae (1999), Biochemistry, 38, 13461-13472.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.2 expressed in Escherichia coli CC118 Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
7.2.4.2 C87A slightly increased activity Klebsiella pneumoniae
7.2.4.2 C87A/A194C no effect on activity Klebsiella pneumoniae
7.2.4.2 C87A/I198C no effect on activity Klebsiella pneumoniae
7.2.4.2 C87A/L178C about 50% of activity Klebsiella pneumoniae
7.2.4.2 C87A/L7C no activity Klebsiella pneumoniae
7.2.4.2 C87A/P191C no activity Klebsiella pneumoniae
7.2.4.2 C87A/P205C no effect on activity Klebsiella pneumoniae
7.2.4.2 C87A/P205C only traces of activity Klebsiella pneumoniae
7.2.4.2 C87A/S187C no effect on activity Klebsiella pneumoniae
7.2.4.2 C87A/V129C strongly increased activity Klebsiella pneumoniae
7.2.4.2 C87A/Y182C slightly increased activity Klebsiella pneumoniae
7.2.4.2 additional information fusion protein from different domains of the enzyme subunits Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.2 membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.4.2 Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.2 Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.2 Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.2 Oxaloacetate reaction is catalyzed at low rate by the alpha subunit alone, much higher activity with the complete enzyme Klebsiella pneumoniae Pyruvate + CO2
-
 ?
7.2.4.2 Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.2 trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.2 biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae