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Literature summary extracted from

  • Boehlein, S.K.; Stewart, J.D.; Walworth, E.S.; Thirumoorthy, R.; Richards, N.G.J.; Schuster, S.M.
    Kinetic mechanism of Escherichia coli asparagine synthetase B (1998), Biochemistry, 37, 13230-13238.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 AMP-PNP competitive vs. ATP, noncompetitive vs. aspartate, uncompetitive vs. glutamine Escherichia coli
6.3.5.4 asparagine noncompetitive vs. ATP and aspartate Escherichia coli
6.3.5.4 beta-methylaspartate noncompetitive vs. ATP, competitive vs. aspartate, noncompetitive vs. glutamine Escherichia coli
6.3.5.4 diphosphate noncompetitive vs. ATP Escherichia coli
6.3.5.4 L-glutamic acid gamma-methylester uncompetitive vs. ATP, competitive vs. glutamine Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine Escherichia coli
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 Escherichia coli asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine Escherichia coli
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine
-
 Escherichia coli AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 AS-B
-
 Escherichia coli
6.3.5.4 Asparagine synthetase B
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.5.4 0.91
-
 AMP-PNP
-
 Escherichia coli
6.3.5.4 6.6
-
 L-glutamic acid gamma-methylester
-
 Escherichia coli
6.3.5.4 18
-
 beta-methylaspartate
-
 Escherichia coli