BRENDA - Enzyme Database

Molecular specificity of a medium chain acyl-CoA synthetase for substrates and inhibitors: conformational analysis

Kasuya, F.; Yamaoka, Y.; Igarashi, K.; Fukui, M.; Biochem. Pharmacol. 55, 1769-1775 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
-
Bos taurus
6.2.1.2
2-hydroxyphenylacetic acid
-
Bos taurus
6.2.1.2
diflunisal
competitive
Bos taurus
6.2.1.2
Nalidixic acid
competitive
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.2
mitochondrion
-
Bos taurus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.2
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.2
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.2
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 1-anthracenecarboxylic acid + CoA
2% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + anthracene-1-carboxyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 1-naphthoic acid + CoA
3% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 1-naphthoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 1-naphthylacetic acid + CoA
18% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 1-naphthyl-acetyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 2-anthracenecarboxylic acid + CoA
16% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + anthracene-2-carboxyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 2-naphthylacetic acid + CoA
21% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 2-naphthyl-acetyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-aminobenzoate + CoA
5% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-aminobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-chlorobenzoate + CoA
12% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-chlorobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-methoxybenzoate + CoA
48% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-methoxybenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-methylbenzoate + CoA
23% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-methylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-aminobenzoate + CoA
3% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-aminobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-chlorobenzoate + CoA
17% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-chlorobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-heptylbenzoate + CoA
66% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-heptylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-methoxybenzoate + CoA
47% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-methoxybenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-methylbenzoate + CoA
59% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-methylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + benzoate + CoA
19% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + benzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + cyclohexanoic acid + CoA
41% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + cyclohexanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + dodecanoate + CoA
39% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + dodecanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + hexanoate + CoA
-
649783
Bos taurus
AMP + diphosphate + hexanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + indomethacin + CoA
6% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + ?
-
649783
Bos taurus
?
6.2.1.2
ATP + tranexamic acid + CoA
7% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + tranexoyl-CoA
-
649783
Bos taurus
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.2.1.2
0.0008
-
diflunisal
pH 8.5
Bos taurus
6.2.1.2
0.0044
-
2-hydroxydodecanoic acid
pH 8.5
Bos taurus
6.2.1.2
0.0123
-
Nalidixic acid
pH 8.5
Bos taurus
6.2.1.2
0.37
-
2-hydroxyphenylacetic acid
pH 8.5
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
-
Bos taurus
6.2.1.2
2-hydroxyphenylacetic acid
-
Bos taurus
6.2.1.2
diflunisal
competitive
Bos taurus
6.2.1.2
Nalidixic acid
competitive
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.2.1.2
0.0008
-
diflunisal
pH 8.5
Bos taurus
6.2.1.2
0.0044
-
2-hydroxydodecanoic acid
pH 8.5
Bos taurus
6.2.1.2
0.0123
-
Nalidixic acid
pH 8.5
Bos taurus
6.2.1.2
0.37
-
2-hydroxyphenylacetic acid
pH 8.5
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.2
mitochondrion
-
Bos taurus
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.2
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.2
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 1-anthracenecarboxylic acid + CoA
2% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + anthracene-1-carboxyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 1-naphthoic acid + CoA
3% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 1-naphthoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 1-naphthylacetic acid + CoA
18% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 1-naphthyl-acetyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 2-anthracenecarboxylic acid + CoA
16% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + anthracene-2-carboxyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 2-naphthylacetic acid + CoA
21% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 2-naphthyl-acetyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-aminobenzoate + CoA
5% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-aminobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-chlorobenzoate + CoA
12% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-chlorobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-methoxybenzoate + CoA
48% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-methoxybenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 3-methylbenzoate + CoA
23% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 3-methylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-aminobenzoate + CoA
3% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-aminobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-chlorobenzoate + CoA
17% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-chlorobenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-heptylbenzoate + CoA
66% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-heptylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-methoxybenzoate + CoA
47% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-methoxybenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + 4-methylbenzoate + CoA
59% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + 4-methylbenzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + benzoate + CoA
19% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + benzoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + cyclohexanoic acid + CoA
41% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + cyclohexanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + dodecanoate + CoA
39% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + dodecanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + hexanoate + CoA
-
649783
Bos taurus
AMP + diphosphate + hexanoyl-CoA
-
649783
Bos taurus
?
6.2.1.2
ATP + indomethacin + CoA
6% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + ?
-
649783
Bos taurus
?
6.2.1.2
ATP + tranexamic acid + CoA
7% of the activity with hexanoic acid
649783
Bos taurus
AMP + diphosphate + tranexoyl-CoA
-
649783
Bos taurus
?