BRENDA - Enzyme Database show

Dihydrodipicolinate synthase is not inhibited by its substrate, (S)-aspartate beta-semialdehyde

Dobson, R.C.; Gerrard, J.A.; Pearce, F.G.; Biochem. J. 377, 757-762 (2004)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.3.3.7
biotechnology
enzyme is a target for herbicide and anti-microbial action
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
additional information
no inhibition by (S)-aspartate 4-semialdehyde, but inhibition occurs by a derivative that is built of the compounbd in the enzyme preparation by ozonolysis
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.11
-
(S)-aspartate 4-semialdehyde
pH 8.0, 30°C, with pyruvate
Escherichia coli
4.3.3.7
0.21
-
(R,S)-aspartate 4-semialdehyde
pH 8.0, 30°C, with pyruvate
Escherichia coli
4.3.3.7
0.25
-
pyruvate
pH 8.0, 30°C, with (S)-aspartate 4-semialdehyde
Escherichia coli
4.3.3.7
0.3
-
pyruvate
pH 8.0, 30°C, with (R,S)-aspartate 4-semialdehyde
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
31270
-
mass spectrometry
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Escherichia coli
first step in the biosynthesis of lysine, overview
dihydrodipicolinate + H2O
-
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Escherichia coli XL1-Blue
first step in the biosynthesis of lysine, overview
dihydrodipicolinate + H2O
-
Escherichia coli XL1-Blue
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Escherichia coli
-
expressing the enzyme from a plasmid, gene dapA
-
4.3.3.7
Escherichia coli XL1-Blue
-
expressing the enzyme from a plasmid, gene dapA
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
enzyme expressed from plasmid in strain XL1-Blue, 69fold
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
1.11
-
purified enzyme
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
649771
Escherichia coli
dihydrodipicolinate + H2O
-
649771
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
first step in the biosynthesis of lysine, overview
649771
Escherichia coli
dihydrodipicolinate + H2O
-
649771
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
649771
Escherichia coli XL1-Blue
dihydrodipicolinate + H2O
-
649771
Escherichia coli XL1-Blue
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
first step in the biosynthesis of lysine, overview
649771
Escherichia coli XL1-Blue
dihydrodipicolinate + H2O
-
649771
Escherichia coli XL1-Blue
?
4.3.3.7
additional information
no activity with (R)-aspartate 4-semialdehyde
649771
Escherichia coli
?
-
649771
Escherichia coli
?
4.3.3.7
additional information
no activity with (R)-aspartate 4-semialdehyde
649771
Escherichia coli XL1-Blue
?
-
649771
Escherichia coli XL1-Blue
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
30
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
assay at
Escherichia coli
Application (protein specific)
EC Number
Application
Commentary
Organism
4.3.3.7
biotechnology
enzyme is a target for herbicide and anti-microbial action
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
additional information
no inhibition by (S)-aspartate 4-semialdehyde, but inhibition occurs by a derivative that is built of the compounbd in the enzyme preparation by ozonolysis
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.11
-
(S)-aspartate 4-semialdehyde
pH 8.0, 30°C, with pyruvate
Escherichia coli
4.3.3.7
0.21
-
(R,S)-aspartate 4-semialdehyde
pH 8.0, 30°C, with pyruvate
Escherichia coli
4.3.3.7
0.25
-
pyruvate
pH 8.0, 30°C, with (S)-aspartate 4-semialdehyde
Escherichia coli
4.3.3.7
0.3
-
pyruvate
pH 8.0, 30°C, with (R,S)-aspartate 4-semialdehyde
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
31270
-
mass spectrometry
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Escherichia coli
first step in the biosynthesis of lysine, overview
dihydrodipicolinate + H2O
-
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Escherichia coli XL1-Blue
first step in the biosynthesis of lysine, overview
dihydrodipicolinate + H2O
-
Escherichia coli XL1-Blue
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
enzyme expressed from plasmid in strain XL1-Blue, 69fold
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
1.11
-
purified enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
649771
Escherichia coli
dihydrodipicolinate + H2O
-
649771
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
first step in the biosynthesis of lysine, overview
649771
Escherichia coli
dihydrodipicolinate + H2O
-
649771
Escherichia coli
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
649771
Escherichia coli XL1-Blue
dihydrodipicolinate + H2O
-
649771
Escherichia coli XL1-Blue
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
first step in the biosynthesis of lysine, overview
649771
Escherichia coli XL1-Blue
dihydrodipicolinate + H2O
-
649771
Escherichia coli XL1-Blue
?
4.3.3.7
additional information
no activity with (R)-aspartate 4-semialdehyde
649771
Escherichia coli
?
-
649771
Escherichia coli
?
4.3.3.7
additional information
no activity with (R)-aspartate 4-semialdehyde
649771
Escherichia coli XL1-Blue
?
-
649771
Escherichia coli XL1-Blue
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
assay at
Escherichia coli